| Effect of plant neutrophil elastase inhibitor on leucocyte migration, adhesion and cytokine release in inflammatory conditions. | |
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MedLine Citation:
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PMID: 20860667 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: The serine and cysteine peptidase inhibitor, BbCI, isolated from Bauhinia bauhinioides seeds, is similar to the classical plant Kunitz inhibitor, STI, but lacks disulphide bridges and methionine residues. BbCI blocks activity of the serine peptidases, elastase (K(iapp) 5.3 nM) and cathepsin G (K(iapp) 160.0 nM), and the cysteine peptidase cathepsin L (K(iapp) 0.2 nM). These three peptidases play important roles in the inflammatory process. EXPERIMENTAL APPROACH: We measured the effects of BbCI on paw oedema and on leucocyte accumulation in pleurisy, both induced by carrageenan. Leucocyte-endothelial cell interactions in scrotal microvasculature in Wistar rats were investigated using intravital microscopy. Cytokine levels in pleural exudate and serum were measured by elisa. KEY RESULTS: Pretreatment of the animals with BbCI (2.5 mg·kg(-1)), 30 min before carrageenan-induced inflammation, effectively reduced paw oedema and bradykinin release, neutrophil migration into the pleural cavity. The number of rolling, adhered and migrated leucocytes at the spermatic fascia microcirculation following carrageenan injection into the scrotum were reduced by BbCI pretreatment. Furthermore, levels of the rat chemokine cytokine-induced neutrophil chemo-attractant-1 were significantly reduced in both pleural exudates and serum from animals pretreated with BbCI. Levels of interleukin-1β or tumour necrosis factor-α, however, did not change. CONCLUSIONS AND IMPLICATIONS: Taken together, our data suggest that the anti-inflammatory properties of BbCI may be useful in investigations of other pathological processes in which human neutrophil elastase, cathepsin G and cathepsin L play important roles. |
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Authors:
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C Oliveira; R A Navarro-Xavier; E A Anjos-Vallota; J O Martins; V L F Silveira; L R C Gonçalves; M S Araújo; G Motta; P Sannomiya; M L V Oliva |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of pharmacology Volume: 161 ISSN: 1476-5381 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-23 Completed Date: 2010-12-02 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 899-910 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. British Journal of Pharmacology © 2010 The British Pharmacological Society. |
Affiliation:
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Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, São Paulo, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents / isolation & purification, pharmacology* Bauhinia / chemistry Carrageenan Cathepsin G / antagonists & inhibitors, metabolism Cathepsin L / antagonists & inhibitors, metabolism Cell Adhesion / drug effects Cell Movement / drug effects Cytokines / metabolism Disease Models, Animal Edema / drug therapy, physiopathology Enzyme-Linked Immunosorbent Assay Humans Inflammation / drug therapy*, physiopathology Leukocyte Elastase / antagonists & inhibitors, metabolism Leukocytes / drug effects, metabolism Male Microscopy / methods Plant Proteins / isolation & purification, pharmacology* Rats Rats, Wistar Seeds |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/BbCI protein, Bauhinia bauhinoides; 0/Cytokines; 0/Plant Proteins; 9000-07-1/Carrageenan; EC 3.4.21.20/Cathepsin G; EC 3.4.21.37/Leukocyte Elastase; EC 3.4.22.15/Cathepsin L |
| Comments/Corrections | |
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