Document Detail


Effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields.
MedLine Citation:
PMID:  20363788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial communities in the rhizosphere are dynamic and susceptible to changes in plant conditions. Among the bacteria, the betaproteobacteria play key roles in nutrient cycling and plant growth promotion, and hence the dynamics of their community structures in the rhizosphere should be investigated. Here, the effects of plant cultivar, growth stage, and soil type on the communities associated with potato cultivars Aveka, Aventra, Karnico, Modena, Premiere, and Désirée were assessed for two different fields containing sandy soil with either a high or low organic compound content. Thus, bacterial and betaproteobacterial PCR-denaturing gradient gel electrophoresis analyses were performed to analyze the effects of plant cultivar and growth on the rhizosphere community structure. The analyses showed that in both fields all cultivars had a rhizosphere effect on the total bacterial and betaproteobacterial communities. In addition, the plant growth stage strongly affected the betaproteobacterial communities in both fields. Moreover, the community structures were affected by cultivar, and cultivars differed in physiology, as reflected in their growth rates, root development, and estimated tuber starch contents. Analyses of betaproteobacterial clone libraries constructed for two selected cultivars (one cultivar that produced low-starch-content tubers and one cultivar that produced high-starch-content tubers), as well as bulk soil, revealed that the rhizospheres of the two cultivars selected for specific bacteria, including plant-growth-promoting bacteria, such as Variovorax and Achromobacter spp. In addition, quantitative PCR-based quantification of the Variovorax paradoxus-specific functional gene asfA (involved in desulfonation) indicated that there were clear potato rhizosphere effects on the abundance of this gene. Interestingly, both cultivar type and plant growth stage affected the community under some circumstances.
Authors:
Ozgül Inceoğlu; Joana Falcão Salles; Leo van Overbeek; Jan Dirk van Elsas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-02
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-09-28     Revised Date:  2011-03-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3675-84     Citation Subset:  IM    
Affiliation:
Department of Microbial Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, Kerklaan 30, Haren, Netherlands.
Data Bank Information
Bank Name/Acc. No.:
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MeSH Terms
Descriptor/Qualifier:
Betaproteobacteria / classification*,  genetics,  isolation & purification*
Biodiversity*
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Nucleic Acid Denaturation
Phylogeny
Plant Roots / microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Solanum tuberosum / genetics*,  growth & development*,  microbiology
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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