| Effect of pioglitazone on L-NAME induced hypertension in diabetic rats. | |
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MedLine Citation:
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PMID: 16168716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study investigates the effect of pioglitazone treatment on blood pressure, vascular reactivity and antioxidant enzymes in L-NAME induced hypertension in normal and STZ-diabetic rats. Diabetes was induced in male Sprague Dawley rats (200+/-15 g) by single intravenous injection of 55 mg/kg of streptozotocin (STZ). Rats were randomized into diabetic and nondiabetic groups, Nomega-nitro-L-arginine-methyl ester (L-NAME, 50 mg/kg) was administered in drinking water for 4 weeks. They were treated with pioglitazone (10 mg/kg/day, p.o.) for 4 weeks and following protocol was carried out. Blood pressure, blood glucose levels and body weight were measured. Thoracic aorta was isolated and dose response curve of phenylephrine (PE) with intact and denuded endothelium was recorded. Dose response curve of acetylcholine (Ach) and sodium nitroprusside (SNP) was recorded in precontracted rings. Lipid peroxidation, superoxide dismutase, catalase, and reduced glutathione were estimated in liver, kidney, and aorta. Pioglitazone produced no significant effect on blood glucose levels, body weight and blood pressure of L-NAME administered nondiabetic and diabetic rats. Pioglitazone treatment had no significant effect on PE induced contraction and Ach induced relaxation in L-NAME diabetic and nondiabetic rats. SNP completely relaxed aortic rings of all the groups. Higher oxidative stress in case of diabetic rats was significantly (p<0.05) reduced by pioglitazone treatment. Although pioglitazone reduced oxidative stress in diabetic rats, there was no significant effect on blood pressure as there was complete absence of nitric oxide due to administration of L-NAME. Hence from the present study it can be concluded that reduction in blood pressure in case of STZ-diabetic rats is nitric oxide mediated. |
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Authors:
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Jayesh B Majithiya; Arvind N Parmar; Chitrang J Trivedi; R Balaraman |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-15 |
Journal Detail:
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Title: Vascular pharmacology Volume: 43 ISSN: 1537-1891 ISO Abbreviation: Vascul. Pharmacol. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-11-08 Completed Date: 2006-02-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101130615 Medline TA: Vascul Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 260-6 Citation Subset: IM |
Affiliation:
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Pharmacy Department, Faculty of Technology and Engineering, M. S. University of Baroda, Kalabhavan, Baroda 390001, Gujarat, India. jayeshbm@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Thoracic / drug effects Blood Glucose / metabolism Blood Pressure / drug effects Body Weight / drug effects Catalase / metabolism Diabetes Mellitus, Experimental / physiopathology* Enzyme Inhibitors / pharmacology* Glutathione / metabolism Hypertension / chemically induced*, prevention & control* Hypoglycemic Agents / pharmacology* Lipid Peroxidation / drug effects Male Muscle Contraction / drug effects Muscle, Smooth, Vascular / drug effects NG-Nitroarginine Methyl Ester / antagonists & inhibitors*, pharmacology* Nitric Oxide Synthase Type III / antagonists & inhibitors* Oxidation-Reduction Rats Rats, Sprague-Dawley Superoxide Dismutase / metabolism Thiazolidinediones / pharmacology* Vasoconstrictor Agents / pharmacology Vasodilator Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Enzyme Inhibitors; 0/Hypoglycemic Agents; 0/Thiazolidinediones; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 111025-46-8/pioglitazone; 50903-99-6/NG-Nitroarginine Methyl Ester; 70-18-8/Glutathione; EC 1.11.1.6/Catalase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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