Document Detail


Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells.
MedLine Citation:
PMID:  18647090     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim of the study was to test the effect of photodynamic therapy on fibroblast proliferation in vitro using protoporphyrin IX as a photosensitizer. BACKGROUND DATA: The abnormal proliferation of synovial tissue serves as a propagator of immune response and tissue damage in rheumatoid arthritis. Since the synovial fibroblasts mediate most relevant pathways of joint destruction, they constitute an important target for novel therapeutic approaches. Photodynamic therapy, which is the clinically applied treatment for various tumors, as well as for some non-oncologic diseases, is based on administration of an exogenous photosensitizer which is used to induce cell death of fibroblast cells. MATERIALS AND METHODS: The photosensitizing effects of protoporphyrin IX (PpIX) were studied in the 3T3 cell line that served as a model of fibroblasts and HeLa cells cultured in vitro. RESULTS: The number of fibroblasts and HeLa cells treated with photo-activated PpIX decreased significantly. The compound affects the viability of study cells, causing necrosis of 3T3 cells and apoptosis of HeLa cells. CONCLUSIONS: The light-activated protoporphyrin affected proliferation of both 3T3 and HeLa cells. No effects of the phototherapy were seen in the form of apoptosis of 3T3 cells, whereas the induction of cell death in HeLa cells was detected.
Authors:
Anna Jankowska; Pawel Wiecek; Beata Burczynska
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  26     ISSN:  1557-8550     ISO Abbreviation:  -     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-29     Completed Date:  2009-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  343-7     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, University of Medical Sciences, Rokietnicka, Poznan, Poland. ajanko@amp.edu.pl
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Cell Line
Cell Proliferation / drug effects*
Female
Fibroblasts / drug effects*
Hela Cells / drug effects*
Humans
Mice
Photochemotherapy
Photosensitizing Agents / pharmacology*
Protoporphyrins / pharmacology*
Chemical
Reg. No./Substance:
0/Photosensitizing Agents; 0/Protoporphyrins; 553-12-8/protoporphyrin IX

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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