| Effect of the phosphodiesterase 4 inhibitor, rolipram, on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 cell line. | |
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MedLine Citation:
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PMID: 12615143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have evaluated effects of a phosphodiesterase (PDE) 4 inhibitor on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 clone 8 (10T1/2) cell line. 10T1/2 cells were cultured in minimum essential medium (MEM) and 10% fetal bovine serum with or without 1 microM retinoic acid and/or the PDE 4 inhibitor, rolipram, and harvested at specific intervals before measurement of alkaline phosphatase activity, cAMP production in response to parathyroid hormone, osteocalcin synthesis and expression, and phosphodiesterase activity. Retinoic acid-increased alkaline phosphatase activity, and slightly enhanced cAMP production in response to parathyroid hormone. However, it did not affect osteocalcin synthesis and expression. In the presence of retinoic acid, PDE 4 activity was not changed. A PDE 4 inhibitor, rolipram, and cAMP analog, 8-bromo-cAMP dramatically increased retinoic acid's ability to induce alkaline phosphatase activity. This is the first report that PDE 4 may be involved in regulation of retinoic acid-increased alkaline phosphatase activity. |
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Authors:
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Taku Murata; Toshifumi Sugatani; Kasumi Shimizu; Vincent C Manganiello; Toshiro Tagawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of oral biology Volume: 48 ISSN: 0003-9969 ISO Abbreviation: Arch. Oral Biol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-03-04 Completed Date: 2003-06-26 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: England |
Other Details:
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Languages: eng Pagination: 63-7 Citation Subset: D; IM |
Affiliation:
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Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu, 514-8507, Mie, Japan. muratat@clin.medic.mie-u.ac.jp <muratat@clin.medic.mie-u.ac.jp> |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3',5'-Cyclic-AMP Phosphodiesterases
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antagonists & inhibitors,
metabolism,
physiology Alkaline Phosphatase / drug effects*, metabolism Animals Cell Line Cyclic AMP / biosynthesis Cyclic Nucleotide Phosphodiesterases, Type 4 Drug Synergism Fibroblasts / drug effects*, enzymology Mice Osteocalcin / biosynthesis, genetics Parathyroid Hormone / pharmacology Phosphodiesterase Inhibitors / pharmacology* Rolipram / pharmacology* Tretinoin / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Parathyroid Hormone; 0/Phosphodiesterase Inhibitors; 104982-03-8/Osteocalcin; 302-79-4/Tretinoin; 60-92-4/Cyclic AMP; 61413-54-5/Rolipram; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases; EC 3.1.4.17/Cyclic Nucleotide Phosphodiesterases, Type 4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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