Document Detail

Effect of phorbol esters on iron uptake in human hematopoietic cell lines.
MedLine Citation:
PMID:  6091866     Owner:  NLM     Status:  MEDLINE    
We have investigated the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on iron uptake into human hematopoietic cell lines K562, U937, and HL-60. TPA inhibited both cell growth and iron uptake by these cell lines. This effect was rapid, which is typical of phorbol esters which are biologically active, and it occurred at very low concentrations of TPA. This effect of TPA was dependent upon an inhibition of the transferrin-binding capacity as estimated on intact cells. However, experiments with transferrin binding on cell samples dissolved in 1% Triton X-100 showed that TPA-treated cells exhibited a transferrin-binding capacity similar to that of control cells. On the basis of this result, it is suggested that TPA modified a part of transferrin receptors present in the cells; as a result of this modification, these receptors became unavailable for binding transferrin, but they remained physically present in the cell. Other compounds capable of inducing the differentiation of leukemic cells, such as dimethyl sulfoxide, butyrate, retinoic acid, and 1 alpha,25-dihydroxy-vitamin D3, did not acutely inhibit iron uptake. We also investigated the effect of TPA on transferrin receptors in a cellular system in which phorbol esters stimulate cell proliferation. At 16 X 10(-9) M, TPA markedly stimulated the proliferation of T-lymphocytes. However, in spite of this marked stimulation of cell proliferation, TPA-stimulated lymphocytes exhibited a transferrin-binding capacity much inferior to cells stimulated by other mitogens, such as phytohemagglutinin.
U Testa; M Titeux; F Louache; P Thomopoulos; H Rochant
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  44     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-11-28     Completed Date:  1984-11-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4981-6     Citation Subset:  IM    
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MeSH Terms
Biological Transport / drug effects
Calcifediol / pharmacology
Calcitriol / pharmacology
Cell Line
Dimethyl Sulfoxide / pharmacology
Heme / pharmacology
Iron / metabolism*
Iron Radioisotopes / diagnostic use
Leukemia, Myeloid, Acute
Phorbol Esters / pharmacology*
Phorbols / pharmacology*
Receptors, Cell Surface / metabolism
Receptors, Transferrin
Structure-Activity Relationship
Tetradecanoylphorbol Acetate / pharmacology*
Transferrin / metabolism
Tretinoin / pharmacology
Reg. No./Substance:
0/Iron Radioisotopes; 0/Phorbol Esters; 0/Phorbols; 0/Receptors, Cell Surface; 0/Receptors, Transferrin; 11096-37-0/Transferrin; 14875-96-8/Heme; 16561-29-8/Tetradecanoylphorbol Acetate; 19356-17-3/Calcifediol; 302-79-4/Tretinoin; 32222-06-3/Calcitriol; 67-68-5/Dimethyl Sulfoxide; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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