| Effect of phenobarbitone on the nucleocytoplasmic transport of ribonucleic acid in vitro. | |
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MedLine Citation:
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PMID: 949348 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transport of nucleic acids from the nucleus to the cytoplasm is a potential site for modification of normal cellular processes by drugs and hormones. In this study the effect of phenobarbitone on nucleocytoplasmic transport of ribosomes was measured in an assay system in vitro. The transport of radioactive ribosomes from isolated rat hepatic nuclei to unlabelled post-microsomal supernatant was measured in rats treated with 80 mg of phenobarbitone/kg body wt. or saline 3h before death. With either treatment, transport was linear with time, and dependent on temperature and the presence of ATP. However, phenobarbitone treatment increased transport of ribonucleoproteins over saline-treated animals nearly twofold. The effect of phenobarbitone was mediated through the cytosol, but was not the result of altered stability of the RNA transported to the cytosol. Cycloheximide (5 mg/kg body wt.) given 3.5 h before death inhibited the stimulation of transport by phenobarbitone. The data indicate that phenobarbitone increased the transport of RNA by stimulating the synthesis of cytosol factors that regulate transport of RNA from the nucleus. |
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Authors:
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N Hazan; R McCauley |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 156 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1976 Jun |
Date Detail:
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Created Date: 1976-10-01 Completed Date: 1976-10-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 665-70 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport / drug effects Cell Nucleus / drug effects*, metabolism Cycloheximide / pharmacology Cytosol / drug effects, metabolism Dactinomycin / pharmacology Liver / drug effects, metabolism Male Nucleoproteins / metabolism Phenobarbital / pharmacology* RNA, Ribosomal / metabolism* Rats |
| Chemical | |
Reg. No./Substance:
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0/Nucleoproteins; 0/RNA, Ribosomal; 50-06-6/Phenobarbital; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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