| Effect of perioperative antioxidant therapy on suboptimal islet transplantation in rats. | |
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MedLine Citation:
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PMID: 15808599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Islet transplantation success is limited by the posttransplant inflammatory response, and we are investigating the ability of antioxidants to neutralize this islet damage. We have shown that pyruvate can enhance the engraftment and functionality of a suboptimal islet mass in rats. The present study further investigated the effects of pyruvate, as well as the antioxidants vitamin E and vitamin C. In study A, 350 syngeneic islets were transplanted into the liver of chemically diabetic rats. Antioxidant treatment, or vehicle, was administered during the perioperative period and an intraperitoneal glucose tolerance test (IPGTT) was performed 2 months posttransplant. In study B, 500 syngeneic islets were transplanted under the kidney capsule of chemically diabetic rats. Antioxidant treatment was administered during the perioperative period. Islet-bearing kidney grafts were harvested 24, 48, and 96 hours posttransplant for histological study. Results revealed that pyruvate was the only significantly effective treatment in enhancing the engraftment and functionality of a suboptimal islet mass. Respectively, 56% and 80% of pyruvate-treated rats became normoglycemic after islet transplantation in study A and study B and had a normal insulin response to IPGTT. Histology results from the islet-bearing kidneys were inconclusive as to whether or not pyruvate has an antiapoptotic effect. We conclude that pyruvate, but not vitamin E or vitamin C, aids in the engraftment and functionality of a suboptimal islet mass with as much effectiveness as a full mass in this study. Further investigation into the mechanism of pyruvate protection is still warranted. |
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Authors:
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M L Brown; M Braun; L Cicalese; C Rastellini |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Transplantation proceedings Volume: 37 ISSN: 0041-1345 ISO Abbreviation: Transplant. Proc. Publication Date: 2005 Jan-Feb |
Date Detail:
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Created Date: 2005-04-05 Completed Date: 2005-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 217-9 Citation Subset: IM |
Affiliation:
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University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA. melissa.brown@umassmed.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / therapeutic use* Ascorbic Acid / therapeutic use* Blood Glucose / metabolism Diabetes Mellitus, Experimental / blood, surgery Glucose Tolerance Test Inflammation / prevention & control Insulin / secretion* Intraoperative Care Islets of Langerhans Transplantation / methods*, physiology Rats Rats, Inbred Lew Transplantation, Isogeneic Vitamin E / therapeutic use* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Blood Glucose; 11061-68-0/Insulin; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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