| Effect of pentagastrin on gastric acid secretion in the totally isolated vascularly perfused rat stomach stimulated with the phosphodiesterase inhibitor isobutyl methylxanthine. | |
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MedLine Citation:
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PMID: 2428094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of a very sensitive stomach biomodel, using the totally isolated rat stomach, is reported. Pentagastrin-stimulated acid secretion in anesthetized and conscious rats was also studied, and the capacity to produce acid and the sensitivity towards pentagastrin in the different rat stomach models were compared. The secretory capacity and the sensitivity were evaluated by means of the maximal acid output (MAO) and threshold dose (TD), respectively. Conscious rats provided with gastric fistulas had a MAO of 48 +/- 6.3 mu eq/10 min and a TD of 6 micrograms/kg-h. In anesthetized rats with luminally perfused stomach MAO was reduced to 11.6 +/- 1.2 mu eq/10 min, whereas the sensitivity was increased, as indicated by a TD of 0.5 micrograms/kg-h. Totally isolated vascularly perfused rat stomachs without isobutyl methylxanthine (IMX) produced even less acid (MAO, 2.2 +/- 0.4 mu eq/10 min) without any change in demand for stimulant (TD, 0.25 micrograms/stomach-h). Addition of IMX to the vascular perfusate in the isolated stomach preparation increased the MAO to 8.0 +/- 1.0 mu eq/10 min and induced a remarkable reduction in demand for stimulant, with a TD obtained already at a pentagastrin dose of 0.008 micrograms/stomach-h, corresponding to a concentration of pentagastrin in the vascular perfusate medium of 84.5 pM. Accordingly, the totally isolated vascularly perfused rat stomach stimulated with IMX is the most sensitive bioassay for (penta)gastrin so far reported. |
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Authors:
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P M Kleveland; S E Haugen; H L Waldum |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of gastroenterology Volume: 21 ISSN: 0036-5521 ISO Abbreviation: Scand. J. Gastroenterol. Publication Date: 1986 Jun |
Date Detail:
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Created Date: 1986-10-22 Completed Date: 1986-10-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060105 Medline TA: Scand J Gastroenterol Country: NORWAY |
Other Details:
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Languages: eng Pagination: 577-84 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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pharmacology* Animals Dose-Response Relationship, Drug Gastric Acid / secretion* Gastric Fistula / physiopathology Pentagastrin / pharmacology* Perfusion Rats Stomach / drug effects* Theophylline / analogs & derivatives* |
| Chemical | |
Reg. No./Substance:
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28822-58-4/1-Methyl-3-isobutylxanthine; 5534-95-2/Pentagastrin; 58-55-9/Theophylline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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