Document Detail


Effect of penehyclidine hydrochloride on expressions of MAPK in mice with CLP-induced acute lung injury.
MedLine Citation:
PMID:  20845074     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Penehyclidine hydrochloride (PHC) is a new anticholinergic drug. PHC has been shown to have a good curative effect for sepsis. Mitogen-activated protein kinase (MAPK) has recently been considered to play an important role in sepsis. In this study, the role of MAPK signal pathways in protective effects of PHC preconditioning on acute lung injury in cecal ligation and puncture (CLP)-induced sepsis was investigated. Healthy female mice were randomly divided into 4 groups: sham control, CLP, and 0.3 or 0.45 mg/kg PHC. At 12 h after surgery, arterial blood was drawn for blood gas analysis, and lung tissue samples were collected to examine pulmonary microvascular permeability, IL-6 levels and myeloperoxidase (MPO) activity. MAPK protein expressions were measured using western blot technique. Compared with sham control mice, acute lung injury was induced in CLP group, which was indicated by decreased PaO(2)/FiO(2), increased pulmonary microvascular permeability, IL-6 levels and MPO activity. Furthermore, mice' exposure to CLP induced the increased protein levels of MAPK. Treatment of 0.45 mg/kg PHC markedly improved PaO(2)/FiO(2), decreased pulmonary microvascular permeability, IL-6 levels and MPO activity, and inhibited expressions of extracellular signal-regulated kinase (ERK1/2) and p38 MAPK. Taken together, these results suggest that PHC ameliorated acute lung injury through the inhibition of extracellular signal-regulated kinase (ERK1/2) and p38 MAPK activation in septic mice.
Authors:
Jia Zhan; Yongpan Liu; Zongze Zhang; Chang Chen; Kai Chen; Yanlin Wang
Related Documents :
8964184 - Angiotensin ii mediates mechanical stress-induced cardiac hypertrophy.
22634604 - Inhibition kinetics and regulation of sphingosine kinase 1 expression in prostate cance...
16323984 - Constitutive activation of the 41- and 43-kda mitogen-activated protein (map) kinases i...
23281044 - Hydrosoluble benzo[e]pyridoindolones as potent inhibitors of aurora kinases.
2753884 - Calcium- and lipid-independent protein kinase c autophosphorylation. activation by low ph.
22147914 - Cullin 3 mediates src-3 ubiquitination and degradation to control the retinoic acid res...
Publication Detail:
Type:  Journal Article     Date:  2010-09-16
Journal Detail:
Title:  Molecular biology reports     Volume:  38     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1909-14     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Zhongnan Hospital of Wuhan University, East-lake Road 169, Wuhan, 430071, Hubei, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of dietary betaine supplementation on lipogenesis gene expression and CpG methylation of lipo...
Next Document:  The characterization of transaldolase gene tal from Pichia stipitis and its heterologous expression ...