| Effect of partial proteolysis on the activation energy of beta-n-acetylhexosaminidase precursor and mature forms. | |
| | |
MedLine Citation:
|
PMID: 12705338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Using 3,3'-diclorophenolsulfoftaleinil N-acetyl-beta-D-glucosaminide as a substrate, the apparent activation energy of beta-N-acetylhexosaminidase (Hex) was determined in samples of plasma and urine, as well as in leukocyte and platelet lysates. Incubation with papain produced an increase in this thermodynamic variable for plasma Hex (precursor forms with high molecular mass) that would be caused by the proteolytic action of papain on the Hex A isoenzyme. However, digestion with papain did not significantly modify the activation energy of Hex in leukocyte and platelet lysates (mature enzymatic forms). In 11 healthy subjects and 28 patients with different renal diseases, no statistically significant differences were found with regard to the values obtained in cellular lysates for variations in the activation energy of urinary Hex, regardless of whether they presented normoalbuminuria, microalbuminuria or macroalbuminuria. These results support the hypothesis that even in patients with proteinuria, no significant amounts of plasma Hex precursor forms are found in urine samples, and the source of the enzyme activity is the kidney itself. |
| | |
Authors:
|
Jesús Hermida; J Antonio Casal; J Carlos Tutor |
Related Documents
:
|
7736658 - Involvement of transforming growth factor-beta 1 for platelets-induced stimulation of e... 6195738 - Plasma beta-thromboglobulin levels in lung cancer. 3152928 - Coagulation contact phase factors and inhibitors in beta-thalassemia major children. 9555968 - Platelet protective effect of tak-029, a novel glycoprotein iib/iiia antagonist: an in ... 8972028 - Differential inhibition of fibrinogen binding to agonist- and rgds peptide-activated st... 8840468 - Variable influence of heparin and contrast agents on platelet function as assessed by t... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 41 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2003 Mar |
Date Detail:
|
Created Date: 2003-04-22 Completed Date: 2003-12-03 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
|
Languages: eng Pagination: 302-5 Citation Subset: IM |
Affiliation:
|
Laboratorio Central, Hospital Clinico Universitario, Santiago de Compostela, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blood Platelets
/
enzymology Case-Control Studies Enzyme Activation* Hexosaminidase A Humans Isoenzymes* Kidney Diseases / enzymology Leukocytes / enzymology Papain / metabolism Peptide Hydrolases Thermodynamics beta-N-Acetylhexosaminidases / blood*, urine* |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; EC 3.2.1.52/Hexosaminidase A; EC 3.2.1.52/beta-N-Acetylhexosaminidases; EC 3.4.-/Peptide Hydrolases; EC 3.4.22.2/Papain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genotyping of alcohol dehydrogenase type 2 and 3 using a two-buffer polyacrylamide gel electrophores...
Next Document: Angiogenin and gestational trophoblastic tumors, a promising prognostic marker.