Document Detail


Effect of pH, temperature and media on acid and alkaline phosphatase activity in "clinical" and "nonclinical" isolates of Bordetella bronchiseptica.
MedLine Citation:
PMID:  6722645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Twenty-two isolates of Bordetella bronchiseptica were studied to determine the effects of pH, incubation temperature and type of media on peak acid and alkaline phosphatase activity. The pH optimum for alkaline phosphatase activity was 9.0 for all of the isolates tested. The pH optimum for acid phosphatase activity was 5.8 for 67% of the isolates and 4.8 for 33%. All of the isolates showed peak phosphatase activity at 37 degrees C. No preference was shown in 35% of the isolates between the types of media tested; however, 40% preferred tryptose broth, 20% preferred nutrient broth and 5% preferred brain-heart infusion broth. No relationship was shown between phosphatase activity and the mouse lethality of the isolates.
Authors:
D M Mobley; M M Chengappa; W L Kadel; J G Stuart
Related Documents :
9027995 - Development of a protein phosphatase-based assay for the detection of phosphatase inhib...
406895 - Codominant autosomal inheritance of polymorphic red cell acid phosphates of lemurs and ...
8722085 - Purification of acid phosphatase i from germinating seeds of vigna sinensis.
7364345 - Localization of some enzymes in polycephalic larva of hydatigera krepkogorski (schultz ...
23570785 - 27-nor-Δ(4)-dafachronic acid is a synthetic ligand of caenorhabditis elegans daf-12 rec...
23579455 - Functional inhibitors of acid sphingomyelinase (fiasmas).
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of comparative medicine. Revue canadienne de médecine comparée     Volume:  48     ISSN:  0008-4050     ISO Abbreviation:  Can. J. Comp. Med.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-07-23     Completed Date:  1984-07-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0151747     Medline TA:  Can J Comp Med     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  175-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism*
Alkaline Phosphatase / metabolism*
Animals
Bordetella / enzymology*,  pathogenicity
Culture Media
Hydrogen-Ion Concentration
Mice
Respiratory Tract Infections / microbiology,  veterinary*
Swine / microbiology*
Swine Diseases / microbiology*
Temperature
Chemical
Reg. No./Substance:
0/Culture Media; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.2/Acid Phosphatase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of Pasteurella multocida isolated from rabbits in Canada.
Next Document:  The activated partial thromboplastin time of diluted plasma: variability due to the method of fibrin...