| Effect of pH and phosphate on trapping capacity of various heavy metal ions with ferritin reactor in flowing seawater. | |
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MedLine Citation:
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PMID: 16118467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a protein reactor consisting of native liver ferritin of Dasyatis akajei (DALF) and a dialysis bag. Our goal was to study a ferritin reactor for its capacity to trap various heavy metal ions (M2+) in flowing seawater. The reactor is sensitive and inexpensive and can be operated by nonprofessional technicians. A positive relationship between the number of trapped M2+ with the DALF reactor and its concentration in the flowing seawater was observed. Both the pH in the medium and the phosphate content within the ferritin cavity strongly affected trapping capacity. It was found that the ferritin released its phosphate compound directly with a shift in pH without the need for releasing reagent, which differs from the phosphate release characteristics of horse spleen ferritin, as previously described. This behavior evidently makes the trapping capacity with the ferritin reactor weaken, indicating that this trapping capacity is tightly connected to its phosphate compound. Our study shows that a self-regulation ability of the ferritin shell rather than its phosphate compound plays an important role in controlling the rate and capacity of trapping M2+. The ferritin reactor was constructed to monitor the contamination level of M2+ in flowing seawater. Our preliminary data along with fieldwork indicate that the DALF reactor is an analytical means for effectively monitoring the contamination level of M2+ in flowing seawater. |
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Authors:
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Bo Kong; He-Qing Huang; Qing-Mei Lin; Zong-Wei Cai; Ping Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 126 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-24 Completed Date: 2005-10-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 133-48 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biotechnology, School of Life Sciences, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dialysis Ferritins / chemistry*, isolation & purification Hydrogen-Ion Concentration Liver / chemistry Metals, Heavy / chemistry*, isolation & purification* Phosphates / chemistry* Seawater / chemistry Skates (Fish) Water Movements Water Pollution, Chemical / analysis Water Purification / methods* |
| Chemical | |
Reg. No./Substance:
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0/Metals, Heavy; 0/Phosphates; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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