Document Detail


Effect of pH on mutagenesis by thiols in Salmonella typhimurium TA102.
MedLine Citation:
PMID:  2671715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mutagenicity of thiol (SH)-containing compounds was tested in Salmonella typhimurium TA102 in the liquid preincubation method. Cysteinyl-glycine (CG), cysteine ethyl ester (CEE), L- and D-penicillamine (PA), cysteine (Cys) and glutathione (GSH) were mutagenic to strain TA102 without metabolic activation. On a molar basis, CG was the most potent mutagen. The mutagenicity of the remaining compounds decreased in the order specified above. The mutagenic response of each thiol-containing compound was a function of the pKa of the thiol group and the pH of the preincubation mixture. This indicates that a thiolate anion, rather than a free thiol, is required for mutagenesis.
Authors:
A A Stark; A Arad; S Siskindovich; D A Pagano; E Zeiger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  224     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-10-06     Completed Date:  1989-10-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  89-94     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Tel-Aviv University, Ramat-Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Cysteine / analogs & derivatives,  toxicity
Dipeptides / toxicity
Glutathione / toxicity
Hydrogen-Ion Concentration
Mutagenicity Tests
Mutagens*
Penicillamine / toxicity
Salmonella typhimurium / genetics*
Sulfhydryl Compounds / toxicity*
Chemical
Reg. No./Substance:
0/Dipeptides; 0/Mutagens; 0/Sulfhydryl Compounds; 19246-18-5/cysteinylglycine; 3411-58-3/ethyl cysteine; 52-67-5/Penicillamine; 52-90-4/Cysteine; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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