Document Detail

Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages.
MedLine Citation:
PMID:  17621441     Owner:  NLM     Status:  MEDLINE    
The effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular pathogen Chlamydophila (Cp.) psittaci was evaluated using a model of Buffalo Green Monkey kidney (BGM) cells and HD11 chicken macrophages as artificial hosts. Firstly, the effect of transferrins on the infectivity of the bacteria was evaluated. Pre-incubation of Cp. psittaci with 0.5 to 5 mg/mL ovoTF prior to infecting BGM cells significantly lowered the infection rate (P < 0.05). For both lactoferrins, the infection rate could only be reduced with 5 mg/mL, albeit not significantly as compared to the infection rate created by the untreated bacteria. Secondly, transferrins were tested for their ability to influence bacterial adhesion and entry in HD11 cells. Maximal non-cytotoxic and non-bactericidal concentrations of 0.05 mg/mL ovoTF and 0.5 mg/mL hLF and bLF were used. Overall, ovoTF was more effective than human and bovine LF in inhibiting bacterial irreversible attachment and cell entry and the latter was accompanied by a dose-dependent reduction of actin recruitment at the bacterial entry site. However, once bacteria had entered HD11 cells, transferrins had apparently no effect on intracellular replication. The present findings suggest a possible role for transferrins and especially ovoTF, in preventing avian Cp. psittaci infections.
Delphine Sylvie Anne Beeckman; Caroline M A D Van Droogenbroeck; Bart J A De Cock; Patrick Van Oostveldt; Daisy C G Vanrompay
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-10
Journal Detail:
Title:  Veterinary research     Volume:  38     ISSN:  0928-4249     ISO Abbreviation:  Vet. Res.     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-07-10     Completed Date:  2007-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309551     Medline TA:  Vet Res     Country:  France    
Other Details:
Languages:  eng     Pagination:  729-39     Citation Subset:  IM    
Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Adhesion / drug effects*
Cell Line
Chlamydia Infections / microbiology,  prevention & control,  veterinary*
Chlamydophila psittaci / drug effects*,  growth & development,  pathogenicity,  physiology*
Conalbumin / pharmacology*
Dose-Response Relationship, Drug
Lactoferrin / pharmacology*
Macrophages / microbiology*
Reg. No./Substance:
0/Lactoferrin; 1391-06-6/Conalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A clinically silent respiratory infection with Chlamydophila spp. in calves is associated with airwa...
Next Document:  Surgery for atrial fibrillation.