| Effect of oversulfated chondroitin-6-sulfate or oversulfated fucoidan in the activation of glutamic plasminogen by tissue plasminogen activator: role of lysine and cyanogen bromide-fibrinogen. | |
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MedLine Citation:
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PMID: 18180617 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fucoidan and chondroitin-6-sulfate were oversulfated using chlorosulfonic acid-pyridine complex and were isolated as the sodium salt. Infrared analysis of oversulfated compounds showed introduction of sulfate groups in new positions, and in-vitro studies of the compounds showed a significant increase in the anticoagulant property. Addition of 28.6 microg/ml oversulfated compound gave a two-fold to four-fold increase in the rate of enhancement of activation of glutamic plasminogen by tissue plasminogen activator using 0.05 mol/l Tris buffer (pH 7.35) containing physiological concentrations of NaCl (0.9%). Under these conditions, unfractionated heparin was not active and the native compounds gave less than 30% enhancement. In the present study, the effect of lysine or cyanogen bromide-treated fibrinogen, alone or in combination with the oversulfated compounds, on the activation of glutamic plasminogen by tissue plasminogen activator was investigated. Addition of 16.2 mmol/l L-lysine gave three-fold to four-fold enhancement of activation, which was further enhanced to five-fold to six-fold by addition of 2.86 microg/ml oversulfated chondroitin-6-sulfate or oversulfated fucoidan. Cyanogen bromide-treated fibrinogen (50 microg/ml) gave a 10-fold enhancement of activation by itself, and addition of 2.86 microg/ml oversulfated compounds amplified this to 15-fold. A 25-fold to 35-fold enhancement of activation of glutamic plasminogen was obtained when 2.86 microg/ml oversulfated compounds were combined with 16.2 mmol/l lysine and 50 microg/ml cyanogen bromide-treated fibrinogen. Dilution studies showed that the amplification of the enhancement of lysine by 2.86 microg/ml oversulfated compound was related to interaction of the cofactors with both glutamic plasminogen and tissue plasminogen activator. |
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Authors:
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Yaneth E Carranza; Dorian Anderson; Vasant Doctor |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis Volume: 19 ISSN: 0957-5235 ISO Abbreviation: Blood Coagul. Fibrinolysis Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-08 Completed Date: 2008-03-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9102551 Medline TA: Blood Coagul Fibrinolysis Country: England |
Other Details:
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Languages: eng Pagination: 60-5 Citation Subset: IM |
Affiliation:
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Chemistry Department, Prairie View A&M University, Texas 77445, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chondroitin Sulfates
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chemistry,
pharmacology* Cyanogen Bromide / chemistry, pharmacology Drug Interactions Fibrinogen / chemistry, physiology Humans Lysine / pharmacology* Plasminogen / drug effects*, physiology Polysaccharides / chemistry, pharmacology* Protein Binding Structure-Activity Relationship Sulfates / chemistry Tissue Plasminogen Activator / drug effects, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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08094//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides; 0/Sulfates; 506-68-3/Cyanogen Bromide; 56-87-1/Lysine; 9001-32-5/Fibrinogen; 9001-91-6/Plasminogen; 9007-28-7/Chondroitin Sulfates; 9072-19-9/fucoidan; EC 3.4.21.68/Tissue Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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