Document Detail

Effect of GRP75/mthsp70/PBP74/mortalin overexpression on intracellular ATP level, mitochondrial membrane potential and ROS accumulation following glucose deprivation in PC12 cells.
MedLine Citation:
PMID:  15724436     Owner:  NLM     Status:  MEDLINE    
Glucose regulated protein 75 (GRP75) is an important molecular chaperon belonged to the heat shock protein (HSP) family. To evaluate the effect of GRP75 overexpression on PC12 cells under glucose deprivation, cell viability and mitochondrial function of GRP75-overexpressing PC12 cells and the vector transfected control PC12 cells were monitored during glucose deprivation. Upon exposure to glucose deprivation, GRP75-overexpressing PC12 cells exhibited more moderate cell damage than control PC12 cells. Both of the two groups of cells showed a decreased ATP level following an early increase in the condition of glucose deprivation, and the mitochondrial potential were also reduced in the similar manner in the two groups of cells. Control PC12 cells showed an immediate and rapid increase in ROS accumulation after the onset of GD treatment, and this accumulation was slowed and reduced in GRP75-overexpressing PC12 cells. These findings suggested that GRP75 could inhibit the ROS accumulation, and it may be associated with the cytoprotective effect of GRP75 overexpression upon glucose deprivation.
Yan Liu; Wen Liu; Xiao-Dong Song; Ji Zuo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  268     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-02-23     Completed Date:  2005-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  45-51     Citation Subset:  IM    
Department of Cellular and Genetic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
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MeSH Terms
Adenosine Triphosphate / metabolism*
Cytoprotection / genetics,  physiology*
Gene Expression
Glucose / deficiency*,  metabolism
HSP70 Heat-Shock Proteins / biosynthesis*,  genetics
Membrane Potentials / genetics,  physiology
Mitochondria / physiology*
PC12 Cells
Reactive Oxygen Species / metabolism*
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Reactive Oxygen Species; 0/mortalin; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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