Document Detail

Effect of ovarian cancer ascites on cell migration and gene expression in an epithelial ovarian cancer in vitro model.
MedLine Citation:
PMID:  20689764     Owner:  NLM     Status:  In-Data-Review    
A third of patients with epithelial ovarian cancer (EOC) present ascites. The cellular fraction of ascites often consists of EOC cells, lymphocytes, and mesothelial cells, whereas the acellular fraction contains cytokines and angiogenic factors. Clinically, the presence of ascites correlates with intraperitoneal and retroperitoneal tumor spread. We have used OV-90, a tumorigenic EOC cell line derived from the malignant ascites of a chemonaive ovarian cancer patient, as a model to assess the effect of ascites on migration potential using an in vitro wound-healing assay. A recent report of an invasion assay described the effect of ascites on the invasion potential of the OV-90 cell line. Ascites sampled from 31 ovarian cancer patients were tested and compared with either 5% fetal bovine serum or no serum for their nonstimulatory or stimulatory effect on the migration potential of the OV-90 cell line. A supervised analysis of data generated by the Affymetrix HG-U133A GeneChip identified differentially expressed genes from OV-90 cells exposed to ascites that had either a nonstimulatory or a stimulatory effect on migration. Ten genes (IRS2, CTSD, NRAS, MLXIP, HMGCR, LAMP1, ETS2, NID1, SMARCD1, and CD44) were upregulated in OV-90 cells exposed to ascites, allowing a nonstimulatory effect on cell migration. These findings were validated by quantitative polymerase chain reaction. In addition, the gene expression of IRS2 and MLXIP each correlated with prognosis when their expression was assessed in an independent set of primary cultures established from ovarian ascites. This study revealed novel candidates that may play a role in ovarian cancer cell migration.
Liliane Meunier; Marie-Line Puiffe; Cécile Le Page; Abdelali Filali-Mouhim; Mario Chevrette; Patricia N Tonin; Diane M Provencher; Anne-Marie Mes-Masson
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Publication Detail:
Type:  Journal Article     Date:  2010-08-01
Journal Detail:
Title:  Translational oncology     Volume:  3     ISSN:  1936-5233     ISO Abbreviation:  Transl Oncol     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101472619     Medline TA:  Transl Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  230-8     Citation Subset:  -    
Centre de recherche du Centre hospitalier de l'Université de Montréal/Institut du cancer de Montréal, Montreal, Quebec, Canada.
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