Document Detail

Effect of ouabain on growth regulation by serum components in Balb/c-3T3 cells: inhibition of entry into S phase by decreased protein synthesis.
MedLine Citation:
PMID:  6257737     Owner:  NLM     Status:  MEDLINE    
The effect of inhibition of the cell membrane Na+-K+ pump on the Balb/c-3T3 cell growth cycle was studied. Inhibition of the Na+-K+ pump resulted in a dose-dependent reduction of intracellular K+ concentration ((K+)i). However, inhibition of protein synthesis in Go/G1 and of subsequent entry into S phase occurred only after (K+)i fell below a critical threshold (50-60 mmoles/liter). Thus, when the (K+)i falls below a critical threshold, protein synthesis is inhibited, preventing cells from entering the S phase. The platelet-derived growth factor (PDGF) induces cells to become "competent" to traverse the cell cycle; the platelet-poor plasma component of serum allows competent cells to progress through G0/G1 and enter S phase. Inhibition of the Na+-K+ pump did not prevent the induction of competence by PDGF, but it did reversibly inhibit plasma-mediated events in early G0/G1. Similarly, cycloheximide inhibited plasma-mediated events but did not prevent PDGF-induced competence. Thus, protein synthesis may not be required for induction of competence; alternatively, the induction of the competent state may occur in these cells after removal of PDGF and protein synthesis inhibitor. Protein synthesis is required for subsequent plasma-mediated events in G0/G1.
C N Frantz; C D Stiles; W J Pledger; C D Scher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  105     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-04-13     Completed Date:  1981-04-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  439-48     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Blood Platelets / physiology
Cell Cycle / drug effects*
Cells, Cultured
DNA Replication / drug effects
Growth Inhibitors
Growth Substances / blood
Ouabain / pharmacology*
Potassium / physiology*
Protein Biosynthesis
Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Growth Inhibitors; 0/Growth Substances; 630-60-4/Ouabain; 7440-09-7/Potassium; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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