| Effect of ouabain on growth regulation by serum components in Balb/c-3T3 cells: inhibition of entry into S phase by decreased protein synthesis. | |
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MedLine Citation:
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PMID: 6257737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of inhibition of the cell membrane Na+-K+ pump on the Balb/c-3T3 cell growth cycle was studied. Inhibition of the Na+-K+ pump resulted in a dose-dependent reduction of intracellular K+ concentration ((K+)i). However, inhibition of protein synthesis in Go/G1 and of subsequent entry into S phase occurred only after (K+)i fell below a critical threshold (50-60 mmoles/liter). Thus, when the (K+)i falls below a critical threshold, protein synthesis is inhibited, preventing cells from entering the S phase. The platelet-derived growth factor (PDGF) induces cells to become "competent" to traverse the cell cycle; the platelet-poor plasma component of serum allows competent cells to progress through G0/G1 and enter S phase. Inhibition of the Na+-K+ pump did not prevent the induction of competence by PDGF, but it did reversibly inhibit plasma-mediated events in early G0/G1. Similarly, cycloheximide inhibited plasma-mediated events but did not prevent PDGF-induced competence. Thus, protein synthesis may not be required for induction of competence; alternatively, the induction of the competent state may occur in these cells after removal of PDGF and protein synthesis inhibitor. Protein synthesis is required for subsequent plasma-mediated events in G0/G1. |
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Authors:
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C N Frantz; C D Stiles; W J Pledger; C D Scher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 105 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1980 Dec |
Date Detail:
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Created Date: 1981-04-13 Completed Date: 1981-04-13 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 439-48 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Animals Blood Platelets / physiology Cell Cycle / drug effects* Cells, Cultured DNA Replication / drug effects Growth Inhibitors Growth Substances / blood Mice Ouabain / pharmacology* Potassium / physiology* Protein Biosynthesis Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors |
| Grant Support | |
ID/Acronym/Agency:
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CA 18662/CA/NCI NIH HHS; CA 22042/CA/NCI NIH HHS; CA 26889/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Growth Inhibitors; 0/Growth Substances; 630-60-4/Ouabain; 7440-09-7/Potassium; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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