Document Detail


Effect of osmotic stress on plant growth promoting Pseudomonas spp.
MedLine Citation:
PMID:  20700582     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we isolated and screened drought tolerant Pseudomonas isolates from arid and semi arid crop production systems of India. Five isolates could tolerate osmotic stress up to -0.73 MPa and possessed multiple PGP properties such as P-solubilization, production of phytohormones (IAA, GA and cytokinin), siderophores, ammonia and HCN however under osmotic stress expression of PGP traits was low compared to non-stressed conditions. The strains were identified as Pseudomonas entomophila, Pseudomonas stutzeri, Pseudomonas putida, Pseudomonas syringae and Pseudomonas monteilli respectively on the basis of 16S rRNA gene sequence analysis. Osmotic stress affected growth pattern of all the isolates as indicated by increased mean generation time. An increase level of intracellular free amino acids, proline, total soluble sugars and exopolysaccharides was observed under osmotic stress suggesting bacterial response to applied stress. Further, strains GAP-P45 and GRFHYTP52 showing higher levels of EPS and osmolytes (amino acids and proline) accumulation under stress as compared to non-stress conditions, also exhibited higher expression of PGP traits under stress indicating a relationship between stress response and expression of PGP traits. We conclude that isolation and screening of indigenous, stress adaptable strains possessing PGP traits can be a method for selection of efficient stress tolerant PGPR strains.
Authors:
V Sandhya; Sk Z Ali; B Venkateswarlu; Gopal Reddy; Minakshi Grover
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-11
Journal Detail:
Title:  Archives of microbiology     Volume:  192     ISSN:  1432-072X     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  867-76     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Central Research Institute for Dryland Agriculture, Santoshnagar, Hyderabad, Andhra Pradesh, India.
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MeSH Terms
Descriptor/Qualifier:
Ammonia / metabolism
India
Indoleacetic Acids / metabolism
Osmosis*
Plant Growth Regulators / metabolism*
Plants / growth & development,  metabolism,  microbiology*
Pseudomonas / growth & development,  isolation & purification,  metabolism*
Siderophores / metabolism
Soil Microbiology*
Stress, Physiological
Chemical
Reg. No./Substance:
0/Indoleacetic Acids; 0/Plant Growth Regulators; 0/Siderophores; 7664-41-7/Ammonia; 87-51-4/indoleacetic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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