Document Detail

Effect of olfactory ensheathing cells on reactive astrocytes in vitro.
MedLine Citation:
PMID:  17447004     Owner:  NLM     Status:  MEDLINE    
Olfactory ensheathing cells have been used in several studies to promote repair in the injured spinal cord. However, cellular interaction between olfactory ensheathing cells and glial cells induced to be reactive in the aftermath of injury site has not been investigated. Using an in vitro model of astrogliosis, we show that reactive astrocytes expressed significantly less glial fibrillary acidic protein (GFAP) when cultured both in direct contact with olfactory ensheathing cells and when the two cell types were separated by a porous membrane. Immunofluorescence staining also suggested that reactive astrocytes showed decreased chondroitin sulfate proteoglycans in the presence of olfactory ensheathing cells, although the reduction was not statistically significant. No down-regulation of GFAP was observed when reactive astrocytes were similarly cultured with Schwann cells. Cell viability assay and bromodeoxyuridine uptake showed that proliferation of reactive astrocytes was significantly increased in the presence of olfactory ensheathing cells and Schwann cells.
D A O'Toole; A K West; M I Chuah
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  64     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1303-9     Citation Subset:  IM    
NeuroRepair Group, University of Tasmania, Hobart, Tasmania, Australia.
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MeSH Terms
Astrocytes / cytology,  metabolism*
Blotting, Western
Cell Communication / physiology
Cell Proliferation
Cell Survival
Cells, Cultured
Chondroitin Sulfates / metabolism
Coculture Techniques
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein / biosynthesis
Neuroepithelial Cells / cytology,  metabolism*
Olfactory Mucosa / cytology,  metabolism*
Schwann Cells / cytology,  metabolism
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 9007-28-7/Chondroitin Sulfates

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