Document Detail


Effect of an o-raffinose cross-linked haemoglobin product on oesophageal and lower oesophageal sphincter motor function.
MedLine Citation:
PMID:  10583849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present experiments evaluate the effects on oesophageal motility of an o-raffinose cross-linked haemoglobin-based oxygen carrier (HBOC) purified from outdated donated human blood cells (HemolinkTM), with attention to dose-response (0.6-2.4 g kg-1), oxygenation status and low molecular weight components (4.4-36.4% 64 kDa or less). In ketamine-anaesthetized cats, lower oesophageal sphincter (LES) function and oesophageal peristalsis were monitored 0.5 h before, during and up to 3.5 h after HBOC infusion, and in some cats at 24 h. (1) All products significantly inhibited LES relaxation and increased peristaltic velocity in the distal smooth muscle oesophagus, without consistently altering resting LES pressure. (2) Effects on peristaltic velocity reached a maximum at the smallest dose, whereas the effects on LES relaxation had a maximum effect at 1.2 g kg-1. (3) Effects were not significantly altered by the haemoglobin oxygenation status or presence of low molecular weight components. (4) Repetitive oesophageal contractions occurred. In the cat, an o-raffinose cross-linked human haemoglobin product produces changes in oesophageal body and LES function, which are independent of the HBOC oxygenation status and composition of the low molecular weight components tested. Changes may persist for at least 24 h. These motility changes are likely due to scavenging of nitric oxide by the haemoglobin.
Authors:
S Xue; W Paterson; D Valdez; D Miller; B Christoff; L T Wong; N E Diamant
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society     Volume:  11     ISSN:  1350-1925     ISO Abbreviation:  Neurogastroenterol. Motil.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-28     Completed Date:  2000-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9432572     Medline TA:  Neurogastroenterol Motil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  421-30     Citation Subset:  IM    
Affiliation:
University of Toronto, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atropine / pharmacology
Cats
Cross-Linking Reagents
Dose-Response Relationship, Drug
Esophagogastric Junction / drug effects*
Esophagus / drug effects*
Female
Hemoglobins / pharmacology*
Male
Muscarinic Antagonists / pharmacology
Muscle Contraction / drug effects
Muscle Relaxation / drug effects
Muscle, Smooth / drug effects
Oxygen Consumption / drug effects
Peristalsis / drug effects
Raffinose / analogs & derivatives*,  pharmacology
Time Factors
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Hemoglobins; 0/Muscarinic Antagonists; 0/O-raffinose cross-linked human hemoglobin; 51-55-8/Atropine; 512-69-6/Raffinose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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