Document Detail

Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection.
MedLine Citation:
PMID:  23076277     Owner:  NLM     Status:  MEDLINE    
Larvae of Galleria mellonella are widely used to study the virulence of microbial pathogens and for assessing the potency of antimicrobial agents. This work examined the effect of nutritional deprivation on the ability of larvae to withstand infection in order to establish standardized conditions for the treatment of larvae for in vivo testing. Larvae deprived of food for seven days demonstrated an increased susceptibility to infection by the yeast Candida albicans. These larvae displayed a lower density of hemocytes compared with controls but hemocytes from starved and control larvae demonstrated the same ability to kill yeast cells. Hemolymph from starved larvae demonstrated reduced expression of a range of antimicrobial peptides (e.g., lipocalin) and immune proteins (e.g., apolipophorin and arylphorin). Deprivation of G. mellonella larvae of food leads to a reduction in the cellular and immune responses and an increased susceptibility to infection. Researchers utilizing these larvae should ensure adequate food is provided to larvae in order to allow valid comparisons to be made between results from different laboratories.
Nessa Banville; Niall Browne; Kevin Kavanagh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  Virulence     Volume:  3     ISSN:  2150-5608     ISO Abbreviation:  Virulence     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-05     Completed Date:  2013-06-24     Revised Date:  2013-10-09    
Medline Journal Info:
Nlm Unique ID:  101531386     Medline TA:  Virulence     Country:  United States    
Other Details:
Languages:  eng     Pagination:  497-503     Citation Subset:  IM    
Department of Biology, National University of Ireland, Maynooth, Ireland.
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MeSH Terms
Antimicrobial Cationic Peptides / biosynthesis
Apolipoproteins / biosynthesis
Candida albicans / immunology*,  pathogenicity
Candidiasis / immunology*,  microbiology
Food Deprivation
Hemocytes / immunology
Hemolymph / immunology
Insect Proteins / biosynthesis,  metabolism*
Larva / immunology*,  microbiology
Lipocalins / biosynthesis
Moths / immunology*
Reg. No./Substance:
0/Antimicrobial Cationic Peptides; 0/Apolipoproteins; 0/Insect Proteins; 0/Lipocalins; 0/apolipophorin III; 0/storage proteins, Insecta
Comment In:
Virulence. 2012 Oct 1;3(6):474-6   [PMID:  23211681 ]

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