| Effect of normothermic perfusion using fructose-1,6-bisphosphate for maintenance of liver function during in situ extended hepatectomy by the total hepatic vascular exclusion technique. | |
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MedLine Citation:
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PMID: 17084408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Recently, hepatic surgery has made remarkable progress, and it is important to use appropriate liver perfusion. We evaluated the effect of normothermic liver perfusion with the addition of fructose-1, 6-bisphosphate (FBP) and oxygenation to maintain liver parenchymal, non-parenchymal, and Kupffer cell function. MATERIALS AND METHODS: The rats were divided into five groups according to the perfusate and continuous perfusion was performed: Control group = 4 degrees C lactate Ringer with 10% glucose (LRG) solution; normothermic group = 25 degrees C LRG solution; normothermic oxygenated group = 25 degrees C oxygenated LRG solution; normothermic FBP group = 25 degrees C LRG solution with addition of 10 mmol/L FBP; normothermic oxygenated FBP group = 25 degrees C oxygenated LRG solution with addition of 10 mmol/L FBP. Parameters under evaluation were oxygen consumption, liver energy level (adenosine triphosphate, total adenine nucleotide), glutathione, lipid peroxide, hyaluronic acid uptake ratio, apoptosis, and histomorphology. Moreover, we studied the effect of FBP and normothermia on Kupffer cells activation in vitro. RESULTS: Liver energy level was lower in the normothermic group than the control group. But, it was improved by oxidation or addition of FBP, and it was satisfactorily maintained up to 120 min in the group with normothermic oxygenated FBP. Hyaluronic acid uptake was maintained highly at all times as measured in normothermic oxygenated FBP group. The uptake of lipopolysaccharide was significantly higher as a result of adding FBP, compared with that in the control group and the normothermic group. Moreover, the apoptotic index in the liver was decreased in normothermic FBP group compared to control group. CONCLUSIONS: The normothermic liver perfusion under additional FBP and oxygenation protects both parenchymal and non-parenchymal cells from reperfusion injury. |
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Authors:
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Ryuichiro Uemura; Kazuhisa Uchiyama; Satoru Ozawa; Hiroki Yamaue |
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Publication Detail:
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Type: Journal Article Date: 2006-11-02 |
Journal Detail:
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Title: The Journal of surgical research Volume: 137 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-02-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 89-95 Citation Subset: IM |
Affiliation:
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Second Department of Surgery, Wakayama Medical University, School of Medicine, Wakayama, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Apoptosis Energy Metabolism / drug effects Fructosediphosphates / pharmacology* Hepatectomy / methods* Hepatocytes / cytology, metabolism In Situ Nick-End Labeling Isotonic Solutions / pharmacology Kupffer Cells / cytology, metabolism Lipid Peroxidation / drug effects Liver / cytology, metabolism*, surgery* Liver Circulation Male Oxidative Stress / drug effects Oxygen / pharmacology Perfusion / methods* Rats Rats, Sprague-Dawley Recovery of Function / drug effects Temperature |
| Chemical | |
Reg. No./Substance:
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0/Fructosediphosphates; 0/Isotonic Solutions; 488-69-7/fructose-1,6-diphosphate; 56-65-5/Adenosine Triphosphate; 7782-44-7/Oxygen; 8022-63-7/Ringer's lactate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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