Document Detail


Effect of norcantharidin on N-acetyltransferase activity in HepG2 cells.
MedLine Citation:
PMID:  11321474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The inhibition ofarylamine N-acetyltransferase (NAT) activity by norcantharidin (NCTD), the demethylated form of cantharidin, in human hepatocellular carcinoma HepG2 cells was investigated. By using high performance liquid chromatography, NAT activity on acetylation of 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) were examined. Two assay systems were performed, one with cellular cytosols, the other with intact HepG2 cell suspensions. The NAT activity in HepG2 cell line was inhibited by norcantharidin in a dose-dependent manner in both types of examined systems: i.e. the greater the concentration of norcantharidin in the reaction, the greater the inhibition of NAT activities. This report is the first to show that norcantharidin has an inhibitory effect on NAT activity in HepG2 cell.
Authors:
L T Wu; J G Chung; J C Chen; W Tsauer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of Chinese medicine     Volume:  29     ISSN:  0192-415X     ISO Abbreviation:  Am. J. Chin. Med.     Publication Date:  2001  
Date Detail:
Created Date:  2001-04-25     Completed Date:  2001-09-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7901431     Medline TA:  Am J Chin Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  161-72     Citation Subset:  IM    
Affiliation:
Department of Microbiology, China Medical College, Taichung, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
4-Aminobenzoic Acid / metabolism
Acetylation
Antineoplastic Agents / pharmacology*
Arylamine N-Acetyltransferase / antagonists & inhibitors*
Bicyclo Compounds, Heterocyclic / pharmacology*
Carcinogens / metabolism
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drugs, Chinese Herbal*
Enzyme Inhibitors / pharmacology*
Fluorenes / metabolism
Humans
Kinetics
Substrate Specificity
Time Factors
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Bicyclo Compounds, Heterocyclic; 0/Carcinogens; 0/Drugs, Chinese Herbal; 0/Enzyme Inhibitors; 0/Fluorenes; 150-13-0/4-Aminobenzoic Acid; 153-78-6/2-aminofluorene; 5442-12-6/norcantharidin; EC 2.3.1.5/Arylamine N-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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