Document Detail

Effect of non-esterified fatty acids on bovine granulosa cell steroidogenesis and proliferation in vitro.
MedLine Citation:
PMID:  15885439     Owner:  NLM     Status:  MEDLINE    
In high-yielding dairy cows, the negative energy balance (NEB) during the first weeks post partum may influence dominant follicle growth and steroidogenesis. Since non-esterified fatty acid (NEFA) concentrations are elevated during NEB and are shown to be toxic for several cell types, we investigated the individual and combined effects of the three main NEFA's on granulosa cell proliferation and steroidogenesis in vitro. Granulosa cells from large follicles were cultured for two days in serum free medium in the presence of palmitic (C16:0) (PA), stearic (C18:0) (SA) and/or oleic acid (C18:1) (OA). Addition of 150, 300 or 500 microM of PA and SA inhibited cell proliferation (P<0.05) while OA only elicited such an effect at 500 microM (P<0.01). In the combination treatment (150 microM of each fatty acid), cell numbers were also reduced (P<0.01). These inhibitory effects on cell number are partly due to the induction of apoptosis by these NEFA's, as was demonstrated by annexin V-FITC/propidium iodide staining of the granulosa cells. Oestradiol-17beta production was stimulated by all doses of PA, by 300 and 500 microM of SA and by 500 microM of OA (P<0.05). Combined treatment with 150 microM of each fatty acid also stimulated oestradiol-17beta production per 10(4) cells (P<0.05). We can conclude that PA, SA and to a lesser degree OA modulate granulosa cell proliferation and steroidogenesis in vitro. These effects may be involved in the occurrence of ovarian dysfunction during the postpartum period in high-yielding dairy cows.
T Vanholder; J L M R Leroy; A Van Soom; G Opsomer; D Maes; M Coryn; A de Kruif
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-10
Journal Detail:
Title:  Animal reproduction science     Volume:  87     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-11     Completed Date:  2005-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  33-44     Citation Subset:  IM    
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
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MeSH Terms
Apoptosis / drug effects
Cattle / metabolism*
Cell Count
Cell Division / drug effects*
Cells, Cultured
Estradiol / biosynthesis
Fatty Acids, Nonesterified / pharmacology*
Granulosa Cells / cytology,  drug effects*,  metabolism*
Oleic Acid / pharmacology
Palmitic Acid / pharmacology
Progesterone / biosynthesis
Stearic Acids / pharmacology
Steroids / biosynthesis*
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Stearic Acids; 0/Steroids; 112-80-1/Oleic Acid; 50-28-2/Estradiol; 57-10-3/Palmitic Acid; 57-11-4/stearic acid; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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