| Effect of non-essential amino acids on fetal rat pancreatic growth and insulin secretion in vitro. | |
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MedLine Citation:
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PMID: 6795297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fetal rat pancreatic rudiments explanted on day 14 of gestation were grown for 6 days in organ culture in medium containing glucose (5.5 or 16.5 mmol/l) and essential amino acids (3.5 or 13.1 mmol/l). Non-essential amino acids (alanine, aspartic acid, asparagine, glycine, proline and serine) were added to the culture medium in a number of combinations and at a maximum total concentration of 4.0 mmol/l. At the end of the period of culture rudiments were compared for DNA content, insulin concentration and quantitative morphology. The release of insulin from the rudiments was tested during 6-h incubations on day 6 of culture. Enrichment of the culture medium with any combination of non-essential amino acids had a slight or no effect on the growth or cellular composition of the rudiment of insulin release from it. Addition of all the non-essential amino acids to medium containing 16.5 mmol glucose and 13.1 mmol essential amino acids/l caused a dramatic reduction in the net insulin accumulation by the cultured rudiment. Combinations of non-essential amino acids in which one or more were omitted did not have the same effect. These findings suggest that fetal rat pancreas grown in vitro may require both essential and non-essential amino acids for the full expression of insulin biosynthesis and secretion. |
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Authors:
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R D Milner; M de Gasparo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of endocrinology Volume: 91 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 1981 Nov |
Date Detail:
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Created Date: 1982-01-28 Completed Date: 1982-01-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 289-97 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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pharmacology Amino Acids / pharmacology* Animals Asparagine / pharmacology Aspartic Acid / pharmacology Glycine / pharmacology Insulin / secretion* Organ Culture Techniques Pancreas / drug effects, embryology*, secretion Proline / pharmacology Rats Rats, Inbred Strains Serine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 11061-68-0/Insulin; 147-85-3/Proline; 56-40-6/Glycine; 56-41-7/Alanine; 56-45-1/Serine; 56-84-8/Aspartic Acid; 7006-34-0/Asparagine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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