| Effect of nitric oxide on contractions of uterine and cervical tissues from pregnant rats. | |
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MedLine Citation:
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PMID: 15293889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our objective was to evaluate the role of the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway in rat uterine and cervical contractility at mid- and late gestation. Rings of uterus and cervix from Sprague Dawley rats on day 14 of pregnancy (mid-) and day 21 of pregnancy (late) were equilibrated at 2 g passive tension in organ chambers filled with Krebs-Henseleit solution and bubbled with 5% CO2 in air (37 degrees C, pH approximately 7.4). Rings were treated with an inhibitor of outward potassium current, tetraethylammonium, to activate phasic contractions, and the concentration-response relationships to diethylamine/NO and 8-bromo-cGMP (8-br-cGMP) were assessed. Baseline spontaneous activity was significantly higher at term gestation in both uterine and cervical rings compared with mid-gestation. Spontaneous contractile activity was not apparent in cervical rings from rats in mid-gestation, but was persistent after treatment with tetraethylammonium. Oxyhemoglobin did not affect NO-induced inhibition of activation by tetraethylammonium contractile activity in either cervical or uterine tissues in mid- or late gestation. The 8-br-cGMP concentration-dependently inhibited tetraethylammonium-activated contractions that were more pronounced in uterine tissues compared with cervical tissues in both mid- and late gestation. We concluded that activation of the NO-cGMP pathway inhibits both uterine and cervical smooth muscle contractility. Both tissues demonstrated refractoriness to NO at term. |
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Authors:
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T Okawa; Y P Vedernikov; G R Saade; R E Garfield |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology Volume: 18 ISSN: 0951-3590 ISO Abbreviation: Gynecol. Endocrinol. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-08-05 Completed Date: 2004-08-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807913 Medline TA: Gynecol Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 186-93 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Division of Reproductive Sciences, University of Texas Medical Branch, Galveston, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cervix Uteri / drug effects* Cyclic GMP / analogs & derivatives*, pharmacology Diethylamines / pharmacology Dose-Response Relationship, Drug Female Gestational Age Nitric Oxide / pharmacology* Pregnancy Rats Rats, Sprague-Dawley Uterine Contraction / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Diethylamines; 10102-43-9/Nitric Oxide; 31356-94-2/8-bromocyclic GMP; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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