Document Detail


Effect of nickel (ni(2+)) on primary human ovarian granulosa cells in vitro.
MedLine Citation:
PMID:  20021108     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Human ovarian granulosa cells obtained from women undergoing in vitro fertilization were exposed to 15.6, 31.25, 62.5, 125, 250, 500, 1000 muM Ni(2+) for 48 h. To determine the site of action of Ni(2+), the granulosa cells were stimulated to produce progesterone (P) by using maximally stimulating amounts of human chorionic gonadotropin (0.1 IU/ml hCG) or dibutyryl cyclic adenosine monophosphate (1 mM db-cAMP). The luteinizing hormone (LH) analog hCG was chosen because resultant P production requires an intact membrane receptor and db-cAMP was used to test for post LH receptor defects caused by Ni(2+). Progesterone content of the culture medium was determined by radioimmunoassay (RIA), and viability of the cells was measured by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction test. Concentration-dependent depression in both hGC and db-cAMP stimulated P production was seen at 15.625 muM or higher concentration of Ni(2+), which is not cytotoxic on human ovarian granulosa cells. The viability of cells was unaffected up to 31.25 muM and decreased significantly at 62.5 muM. Our results show a dose-related depression in stimulated P production of granulosa cells at a dose that does not induce significant cytotoxic action. These data indicate that the effect of Ni(2+) on P production is not due to cytotoxicity, and the cellular site(s) of inhibitory action appears to be subsequent to the membrane receptor and production of cAMP.
Authors:
Csaba Révész; Zsolt Forgács; Péter Lázár; Szabolcs Mátyás; Klára Rajczy; Ferenc Krizsa; Artúr Bernard; István Gáti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  14     ISSN:  1537-6524     ISO Abbreviation:  Toxicol. Mech. Methods     Publication Date:  2004  
Date Detail:
Created Date:  2009-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  England    
Other Details:
Languages:  eng     Pagination:  287-92     Citation Subset:  -    
Affiliation:
National Institute of Chemical Safety, József Fodor National Center for Public Health, Budapest, Hungary.
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