Document Detail

Effect of the neuroprotective compound SR57746A on nerve growth factor synthesis in cultured astrocytes from neonatal rat cortex.
MedLine Citation:
PMID:  10369466     Owner:  NLM     Status:  MEDLINE    
The neurotrophic factor promoting activity of the neuroprotective compound SR57746A was evaluated in primary cultures of neonatal rat cortical astrocytes by studying the synthesis of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). A concentration- and time-dependent increase of nerve growth factor mRNA was induced by SR57746A (10 nM-1 microM). In these astrocytes, BDNF mRNA contents were increased to a significant but smaller extent, and beta-actin mRNA showed no variation. SR57746A (1 microM) induced increases of both de novo protein translation after 6 h of incubation and NGF release into the extracellular medium after 6-24 h. These effects were preceded by a transient augmentation of junB, c-fos and c-jun mRNA contents. These increases of AP-1 family mRNA were associated with increased nuclear AP-1 binding activity. The results show that SR57746A can increase the synthesis and release of NGF in rat cortical astrocytes. Such effects may contribute to the drug's previously described neuroprotective effects.
C Labie; C Lafon; C Marmouget; P Saubusse; J Fournier; P E Keane; G Le Fur; P Soubrié
Related Documents :
14552876 - Neuroprotection and neuronal dysfunction upon repetitive inhibition of oxidative phosph...
15233376 - Processing, distribution, and function of vgf, a neuronal and endocrine peptide precursor.
16076026 - Novel alternative splicing predicts a truncated isoform of the nmda receptor subunit 1 ...
8744446 - Cellular localization of pan-trk immunoreactivity and trkc mrna in the enteric nervous ...
11034826 - Kinetic proofreading scanning models for eukaryotic translational initiation: the cap a...
9143556 - Target contact regulates expression of synaptotagmin genes in spinal motor neurons in v...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  127     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-09-01     Completed Date:  1999-09-01     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-44     Citation Subset:  IM    
Department of Neuropsychiatry Research, Sanofi Recherche, Toulouse, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Newborn
Astrocytes / drug effects,  metabolism*
Brain-Derived Neurotrophic Factor / biosynthesis,  genetics
Cells, Cultured
Cerebral Cortex / cytology,  drug effects,  metabolism*
Enzyme-Linked Immunosorbent Assay
Naphthalenes / pharmacology*
Nerve Growth Factors / biosynthesis*,  genetics
Neuroprotective Agents / pharmacology*
Precipitin Tests
Protein Biosynthesis
Pyridines / pharmacology*
RNA, Messenger / biosynthesis
Rats, Sprague-Dawley
Single-Strand Specific DNA and RNA Endonucleases / metabolism
Transcription Factor AP-1 / metabolism
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Naphthalenes; 0/Nerve Growth Factors; 0/Neuroprotective Agents; 0/Pyridines; 0/RNA, Messenger; 0/Transcription Factor AP-1; EC Specific DNA and RNA Endonucleases; V8QL94KNQO/xaliproden

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Concentration-dependent isoflurane effects on depolarization-evoked glutamate and GABA outflows from...
Next Document:  Effects of (-)-tertatolol, (-)-penbutolol and (+/-)-pindolol in combination with paroxetine on presy...