Document Detail

Effect of the neuroprotective compound SR57746A on nerve growth factor synthesis in cultured astrocytes from neonatal rat cortex.
MedLine Citation:
PMID:  10369466     Owner:  NLM     Status:  MEDLINE    
The neurotrophic factor promoting activity of the neuroprotective compound SR57746A was evaluated in primary cultures of neonatal rat cortical astrocytes by studying the synthesis of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). A concentration- and time-dependent increase of nerve growth factor mRNA was induced by SR57746A (10 nM-1 microM). In these astrocytes, BDNF mRNA contents were increased to a significant but smaller extent, and beta-actin mRNA showed no variation. SR57746A (1 microM) induced increases of both de novo protein translation after 6 h of incubation and NGF release into the extracellular medium after 6-24 h. These effects were preceded by a transient augmentation of junB, c-fos and c-jun mRNA contents. These increases of AP-1 family mRNA were associated with increased nuclear AP-1 binding activity. The results show that SR57746A can increase the synthesis and release of NGF in rat cortical astrocytes. Such effects may contribute to the drug's previously described neuroprotective effects.
C Labie; C Lafon; C Marmouget; P Saubusse; J Fournier; P E Keane; G Le Fur; P Soubrié
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  127     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-09-01     Completed Date:  1999-09-01     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-44     Citation Subset:  IM    
Department of Neuropsychiatry Research, Sanofi Recherche, Toulouse, France.
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MeSH Terms
Animals, Newborn
Astrocytes / drug effects,  metabolism*
Brain-Derived Neurotrophic Factor / biosynthesis,  genetics
Cells, Cultured
Cerebral Cortex / cytology,  drug effects,  metabolism*
Enzyme-Linked Immunosorbent Assay
Naphthalenes / pharmacology*
Nerve Growth Factors / biosynthesis*,  genetics
Neuroprotective Agents / pharmacology*
Precipitin Tests
Protein Biosynthesis
Pyridines / pharmacology*
RNA, Messenger / biosynthesis
Rats, Sprague-Dawley
Single-Strand Specific DNA and RNA Endonucleases / metabolism
Transcription Factor AP-1 / metabolism
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Naphthalenes; 0/Nerve Growth Factors; 0/Neuroprotective Agents; 0/Pyridines; 0/RNA, Messenger; 0/Transcription Factor AP-1; EC Specific DNA and RNA Endonucleases; V8QL94KNQO/xaliproden

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