Document Detail

Effect of neuronal PC12 cells on the functional properties of intestinal epithelial Caco-2 cells.
MedLine Citation:
PMID:  12843659     Owner:  NLM     Status:  MEDLINE    
The effect of neuronal cells on the functional properties of intestinal epithelial cells was examined by using an in vitro coculture system. Two cell lines, Caco-2 and PC12, were respectively used as intestinal epithelial and enteric neuronal cell models. Coculture of differentiated Caco-2 cells with PC12 caused a significant decrease in the transepithelial electrical resistance (TER) value of the Caco-2 monolayer. The permeability to lucifer yellow (LY) was also significantly increased, suggesting that the tight junction (TJ) of the Caco-2 monolayers was modulated by coculturing with PC12. To identify the TJ-modulating factor presumably secreted from PC12, the effects of the major neurotransmitters on the TER value and LY transport were examined, but no influence was apparent. The TJ-modulating effect of PC12 was prevented by exposing PC12 to cycloheximide, suggesting that new protein synthesis in PC12 was necessary for this regulation.
Hideo Satsu; Tatsuya Yokoyama; Nobumasa Ogawa; Yoko Fujiwara-Hatano; Makoto Shimizu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  67     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-04     Completed Date:  2004-04-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1312-8     Citation Subset:  IM    
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
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MeSH Terms
Caco-2 Cells
Coculture Techniques / methods
Cycloheximide / pharmacology
Intestinal Mucosa / cytology*
Neurons / cytology*,  secretion
Neurotransmitter Agents / pharmacology
PC12 Cells
Paracrine Communication*
Protein Biosynthesis
Proteins / physiology
Tight Junctions / drug effects,  metabolism
Time Factors
Tumor Necrosis Factor-alpha / pharmacology
Reg. No./Substance:
0/Neurotransmitter Agents; 0/Proteins; 0/Tumor Necrosis Factor-alpha; 66-81-9/Cycloheximide

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