Document Detail

Effect of neuroactive agents on cholecystokinin release from the isolated, perfused porcine duodenum.
MedLine Citation:
PMID:  3509961     Owner:  NLM     Status:  MEDLINE    
To elucidate the mechanisms involved in the regulation of CCK release from the small intestine, the effect of neuroactive agents on the release of CCK from the isolated, perfused porcine duodenum has been studied. CCK in the venous effluent was measured by three radio-immunoassays specific for the N-terminus, the tyrosine-o-sulphated region and the C-terminus of CCK-33. Gastrin-releasing peptide (GRP) at concentrations of 10(-10) and 10(-9) M in the arterial line increased the CCK output by about 100 and 500%, respectively, as measured with the assay specific for the sulphated region of CCK. Pilocarpine and physostigmine at concentrations of 10(-5) M reduced the CCK output to 24 and 40% of basal, respectively, and also abolished the GRP-stimulated CCK response. Atropine (10(-6) M) increased CCK output about 100%. The results suggest that both stimulatory and inhibitory nervous impulses may be transmitted to the intestinal CCK cell, mediated by intramural GRP neurons and cholinergic pathways, respectively.
P Cantor; J J Holst; S Knuhtsen; J F Rehfeld
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  130     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1987-10-14     Completed Date:  1987-10-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  627-32     Citation Subset:  IM    
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MeSH Terms
Atropine / pharmacology*
Cholecystokinin / metabolism,  secretion*
Duodenum / physiology*
Gastrin-Releasing Peptide
Peptide Fragments / metabolism
Peptides / pharmacology*
Physostigmine / pharmacology*
Pilocarpine / pharmacology*
Reg. No./Substance:
0/Peptide Fragments; 0/Peptides; 122998-75-8/cholecystokinin 33 (10-20); 51-55-8/Atropine; 57-47-6/Physostigmine; 79955-77-4/cholecystokinin 39; 80043-53-4/Gastrin-Releasing Peptide; 9011-97-6/Cholecystokinin; 92-13-7/Pilocarpine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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