Document Detail


Effect of near ultraviolet and visible light on amoeba.
MedLine Citation:
PMID:  1176544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The near ultraviolet and visible light (VL) impinging at an intensity of 2-5 x 10(2) J s-1 m-2 for 2-5 h kills the mitotic and the early S-phase (0- to 15-min-old) amoebae. At the mid- and late S-period only a fraction of cells are killed by VL and G2 phase cells are quite resistant. Amoebae of all cell cycle stages show a delay in the first mitotic division. DNA synthesis, as measured by [3H]thymidine incorporation, is depressed in the VL-exposed early-S amoebae. A concurrent but temporary inhibition in [3H]leucine incorporation also occurs in these cells. However, no significant change in [3H]uridine incorporation has been found. To localize the site of lethal damage, nuclear transplantation studies were undertaken between the control amoebae and the amoebae treated with VL. The nucleus of a VL-exposed early S-phase cell recovers when transplanted immediately after VL exposure into an enucleate G2 cytoplasm but dies if grafted into an enucleat S-phase cytoplasm. The therapeutic effect of the G2 cytoplasm, although at a lower level, is also evident even when the treated early S-phase nucleus is implanted 20 h later, but not after 48 h, into the G2 cytoplasm. The amoeba cytoplasm shows resistance to VL-irradiation, can accept a control nucleus from any cell cycle stage, and function normally. The G2 nucleus also remains apparently unaffected to VL exposure and can survive when it is transfered to the control cytoplasm of any cell-cycle phase. All these findings are discussed in the light of the possible existence of a repair system against VL-induced damage in the G2-phase amoeba.
Authors:
S Chatterjee; S K Bhattacharjee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cell science     Volume:  19     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1975-12-29     Completed Date:  1975-12-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  117-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amoeba / growth & development,  radiation effects*
Animals
Cell Nucleus / radiation effects
Cytoplasm / radiation effects
DNA / biosynthesis
Dose-Response Relationship, Radiation
Light*
Mitosis / radiation effects
Pinocytosis / radiation effects
Protein Biosynthesis
RNA / biosynthesis
Radiation Effects
Ultraviolet Rays*
Chemical
Reg. No./Substance:
63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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