Document Detail

Effect of n-butyric acid on epithelial cell proliferation of pig colonic mucosa in short-term culture.
MedLine Citation:
PMID:  7555697     Owner:  NLM     Status:  MEDLINE    
Short-chain fatty acids (SCFA) such as acetic, propionic and n-butyric acids produced by hindgut bacteria stimulate gut epithelial cell proliferation through afferent neural and efferent non-neural systemic transmissions beside a probable local mechanism. In the present study, we developed an experimental system using pig hindgut mucosa in short term culture to clarify the mechanism of the local trophic effect of SCFA. Pig mucosal tissue pieces of the distal colon were cultured in RPMI 1640 medium containing glutamine, 20% (v/v) newborn calf serum and n-butyric acid (0, 0.5, 1.0 or 5.0 mmol/L). Crypt cell production rate from 0.5 to 3.5 and from 21 to 24 hours of culture was measured. Butyric acid increased crypt cell production rate of pit distal colon only at 5 mmol/L. The effect of butyric acid did not differ between samples of different length of n-butyric acid exposure. The effect of n-butyric acid in this study resembled to that found in human biopsied specimens of the colon. The present results also indicated that epithelial cells of pig colonic mucosa in short-term culture presented here retained the proliferative activity and the responsiveness to n-butyric acid.
T Sakata; M Adachi; M Hashida; N Sato; T Kojima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DTW. Deutsche tierärztliche Wochenschrift     Volume:  102     ISSN:  0341-6593     ISO Abbreviation:  DTW. Dtsch. Tierarztl. Wochenschr.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-11-20     Completed Date:  1995-11-20     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  7706565     Medline TA:  Dtsch Tierarztl Wochenschr     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  163-4     Citation Subset:  IM    
Department of Basic Sciences, Ishinomaki Senshu University, Japan.
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MeSH Terms
Butyric Acid
Butyric Acids / pharmacology*
Cell Division / drug effects
Colon / cytology,  drug effects*
Culture Techniques
Epithelial Cells
Epithelium / drug effects
Intestinal Mucosa / cytology,  drug effects*
Reg. No./Substance:
0/Butyric Acids; 107-92-6/Butyric Acid

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