Document Detail

Effect of n-3 fatty acids and α-tocopherol on post-thaw parameters and fatty acid composition of bovine sperm.
MedLine Citation:
PMID:  22869241     Owner:  NLM     Status:  MEDLINE    
PURPOSE: This study was designed to determine the combined effects of adding source of n-3 fatty acids (FA) and α-tocopherol (vitamin E, VE) to semen extender on freezability and FA composition of Brown Swiss bull sperm.
METHODS: Semen samples were collected from 6 Brown Swiss bulls and pooled. In the first trial, semen was divided into 12 groups including 4 levels of n-3 FA (0, 1, 10, 100 ng ml(-1)) and 3 levels of VE (0. 0.2, 0.4 mM). Motility, viability and fatty acid composition of sperm were measured.
RESULTS: The treatment of 10 ng ml(-1) n-3 FA and 0.4 mM VE had the best post-thaw sperm characteristics (P < 0.01). In the second trial, sperm lipid composition of this treatment and control (without FA and VE) was determined. Supplementing n-3 fatty acids during cryopreservation increased docosahexaenoic acid (DHA) and the ratio of n-3 to n-6 FA in sperm before freezing and after thawing.
CONCLUSIONS: The results suggest that combining the optimal level of n-3 FA (10 ng ml(-1)) with the highest level of VE tested (0.4 mM) in a semen extender changed the membrane lipid composition and improved freezablity of Brown Swiss bull sperm.
A Towhidi; J E Parks
Publication Detail:
Type:  Journal Article     Date:  2012-08-07
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-08     Completed Date:  2013-05-03     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1051-6     Citation Subset:  IM    
Department of Animal Science, Faculty of Agricultural Science and Engineering, University of Tehran, POBox# 4111, Karaj, Iran.
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MeSH Terms
Cryopreservation / methods*
Docosahexaenoic Acids / analysis,  metabolism
Dose-Response Relationship, Drug
Fatty Acids / analysis*,  chemistry
Fatty Acids, Omega-3 / pharmacology*
Fatty Acids, Omega-6 / analysis,  metabolism
Membrane Lipids / chemistry,  metabolism
Semen Preservation / methods*
Sperm Motility / drug effects
Spermatozoa / chemistry,  drug effects*
alpha-Tocopherol / pharmacology*
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/Membrane Lipids; 25167-62-8/Docosahexaenoic Acids; 59-02-9/alpha-Tocopherol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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