Document Detail

Effect of mutations of murine lens alphaB crystallin on transfected neural cell viability and cellular translocation in response to stress.
MedLine Citation:
PMID:  9821953     Owner:  NLM     Status:  MEDLINE    
We examined the influence of over-expressed native and mutant murine lens alphaB crystallin on the response of a murine neural cell line to heat and ionic strength shock. Native and mutant (F27R and KK174/175LL) murine alphaB crystallin amplicons were subcloned into a Lac-Switch IPTG-inducible RSV promoter eukaryotic vector, and transfected into N1E-115 cells using lipofectin. Expression was induced maximally 8 h after addition of IPTG (optimal final concentration 1 mM) to the medium. Cells grew normally after transfection with native and mutant murine alphaB crystallin. We demonstrated expression of the protein using specific anti-alpha crystallin antibodies. Viability under severe heat and ionic strength stress increased when cells had been transfected with native alphaB crystallin, but not with mutants F27R or KK174/175LL. Heat shock caused translocation of both native and mutant alphaB crystallins into the central region of the cells. These results show that mutations in alphaB crystallin that effect its chaperone-like activity may also influence viability of N1E-115 neural cells under stress, while not influencing the distribution of the protein within the cell.
K E Wiesmann; A Coop; D Goode; H W Hepburne-Scott; M J Crabbe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  438     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-02     Completed Date:  1998-12-02     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
Division of Cell and Molecular Biology, School of Animal and Microbial Sciences, The University of Reading, Berks, UK.
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MeSH Terms
Amino Acid Sequence
Cell Movement
Cell Survival
Crystallins / genetics*,  metabolism
Heat-Shock Response*
Microscopy, Confocal
Molecular Sequence Data
Neurons / cytology*,  physiology
Osmolar Concentration
Sequence Alignment
Tumor Cells, Cultured
Grant Support
//Wellcome Trust
Reg. No./Substance:

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