Document Detail


Effect of mutations in Gag on assembly of immature human immunodeficiency virus type 1 capsids in a cell-free system.
MedLine Citation:
PMID:  11145907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of HIV-1 capsid formation in a cell-free system revealed that capsid assembly occurs via an ordered series of assembly intermediates and requires host machinery. Here we use this system to examine 12 mutations in HIV-1 Gag that others studied previously in intact cells. With respect to capsid formation, these mutations generally produced the same phenotype in the cell-free system as in cells, indicating the cell-free system's high degree of fidelity. Analysis of assembly intermediates reveals that a mutation in the distal region of CA (322 LDeltaS) and truncations proximal to the second cys-his box in NC block multimerization of Gag at early stages in the cell-free capsid assembly pathway. In contrast, mutations in the region of amino acids 56-68 (located in the proximal portion of MA) inhibit assembly at a later point in the pathway. Other mutations, including truncations distal to the first cys-his box in NC and mutations in the distal half of MA (88HDeltaG, 85YDeltaG, Delta104-115, and Delta115-129), do not affect formation of immature capsids in the cell-free system. These data provide new information on the role of different domains in Gag during the early events of capsid assembly.
Authors:
A R Singh; R L Hill; J R Lingappa
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  279     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  257-70     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Department of Physiology, University of California at San Francisco, San Francisco, California, 94117, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Capsid / chemistry,  genetics,  metabolism*
Cell-Free System
Centrifugation, Density Gradient / methods
Gene Products, gag / chemistry,  genetics,  metabolism*
Genes, gag*
HIV Antigens / chemistry,  genetics,  metabolism
HIV-1 / genetics,  metabolism*
Humans
Molecular Sequence Data
Mutation*
Nucleocapsid / chemistry,  genetics,  metabolism
Viral Proteins*
Virus Assembly*
gag Gene Products, Human Immunodeficiency Virus
Grant Support
ID/Acronym/Agency:
R29 AI41881/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Gene Products, gag; 0/HIV Antigens; 0/Viral Proteins; 0/gag Gene Products, Human Immunodeficiency Virus; 0/p17 protein, Human Immunodeficiency Virus Type 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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