| Effect of mutations in Gag on assembly of immature human immunodeficiency virus type 1 capsids in a cell-free system. | |
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MedLine Citation:
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PMID: 11145907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies of HIV-1 capsid formation in a cell-free system revealed that capsid assembly occurs via an ordered series of assembly intermediates and requires host machinery. Here we use this system to examine 12 mutations in HIV-1 Gag that others studied previously in intact cells. With respect to capsid formation, these mutations generally produced the same phenotype in the cell-free system as in cells, indicating the cell-free system's high degree of fidelity. Analysis of assembly intermediates reveals that a mutation in the distal region of CA (322 LDeltaS) and truncations proximal to the second cys-his box in NC block multimerization of Gag at early stages in the cell-free capsid assembly pathway. In contrast, mutations in the region of amino acids 56-68 (located in the proximal portion of MA) inhibit assembly at a later point in the pathway. Other mutations, including truncations distal to the first cys-his box in NC and mutations in the distal half of MA (88HDeltaG, 85YDeltaG, Delta104-115, and Delta115-129), do not affect formation of immature capsids in the cell-free system. These data provide new information on the role of different domains in Gag during the early events of capsid assembly. |
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Authors:
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A R Singh; R L Hill; J R Lingappa |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Virology Volume: 279 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-03-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 257-70 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Physiology, University of California at San Francisco, San Francisco, California, 94117, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Capsid / chemistry, genetics, metabolism* Cell-Free System Centrifugation, Density Gradient / methods Gene Products, gag / chemistry, genetics, metabolism* Genes, gag* HIV Antigens / chemistry, genetics, metabolism HIV-1 / genetics, metabolism* Humans Molecular Sequence Data Mutation* Nucleocapsid / chemistry, genetics, metabolism Viral Proteins* Virus Assembly* gag Gene Products, Human Immunodeficiency Virus |
| Grant Support | |
ID/Acronym/Agency:
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R29 AI41881/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gene Products, gag; 0/HIV Antigens; 0/Viral Proteins; 0/gag Gene Products, Human Immunodeficiency Virus; 0/p17 protein, Human Immunodeficiency Virus Type 1 |
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