| Effect of mutations causing overexpression of RNA polymerase alpha subunit on regulation of virulence factors in Bordetella pertussis. | |
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MedLine Citation:
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PMID: 7961498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In Bordetella pertussis, expression of virulence factors is controlled by the Bvg proteins, which comprise a sensor-regulator two-component signal transduction system. Previously, we described a mutant strain of B. pertussis that had reduced transcription of pertussis toxin and adenylate cyclase toxin genes, while other virulence factors were relatively unaffected. We obtained a B. pertussis clone that repaired the defect in both this strain and an independent mutant strain with a similar phenotype when introduced onto the chromosome by allelic exchange. Further analysis revealed that the mutations were just upstream of the translational start site of the rpoA gene encoding the alpha subunit of RNA polymerase. We confirmed that these mutations were responsible for the mutant phenotype by site-directed mutagenesis. Our hypothesis that these mutations cause an overexpression of rpoA was confirmed by Western immunoblotting and translational fusion analysis. Corroboration of this effect was obtained by overexpressing rpoA on a plasmid in wild-type B. pertussis, which caused the same phenotype as the mutants showed. Conclusions in regard to the identity of the transcription activator of the toxin genes are discussed. |
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Authors:
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N H Carbonetti; T M Fuchs; A A Patamawenu; T J Irish; H Deppisch; R Gross |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 176 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1994-12-27 Completed Date: 1994-12-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7267-73 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/Z26647 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase Toxin* Amino Acid Sequence Base Sequence Bordetella pertussis / enzymology, genetics*, pathogenicity* DNA Repair DNA-Directed RNA Polymerases / biosynthesis, genetics* Down-Regulation Gene Expression Regulation, Bacterial* Molecular Sequence Data Mutagenesis, Site-Directed Mutation Nucleic Acid Conformation Pertussis Toxin* Phenotype Promoter Regions, Genetic / genetics Restriction Mapping Species Specificity Transcription, Genetic Virulence / genetics Virulence Factors, Bordetella / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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AI32946/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adenylate Cyclase Toxin; 0/Virulence Factors, Bordetella; EC 2.4.2.31/Pertussis Toxin; EC 2.7.7.6/DNA-Directed RNA Polymerases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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