| Effect of muscarinic receptor stimulation on release of cysteinyl-leukotrienes and thromboxane B2 from anaphylactic guinea-pig hearts. | |
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MedLine Citation:
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PMID: 3072486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of muscarinic receptor stimulation by infusions of methacholine (6.25 x 10(-8) mol/min or 1.9 x 10(-7) mol/min) into isolated perfused, spontaneously beating sensitized guinea-pig hearts on the anaphylactic release of cysteinyl-leukotrienes (LT) and thromboxane (TX) B2 were investigated. Methacholine increased coronary flow and decreased heart rate under basal conditions. Furthermore, infusions of methacholine (1.9 x 10(-7) mol/min) significantly increased the anaphylactic release of TXB2 as well as of immunoreactive cysteinyl-LT, which were demonstrated by reversed phase high pressure liquid chromatography to consist of a mixture of LTC4, LTD4 and LTE4. Infusions of atropine (1.3 x 10(-7) mol/min) alone did not significantly affect coronary flow and heart rate prior to ovalbumin injection nor anaphylactic release of cysteinyl-LT. The anaphylactic release of TXB2 was, however, significantly decreased in the presence of atropine. Atropine (1.3 x 10(-7) mol/min) infused in addition to methacholine (1.9 x 10(-7) mol/min) abolished the effects of the muscarinic receptor agonist on spontaneous heart rate and significantly antagonized the increase in coronary flow prior to ovalbumin injection. Similarly, the simultaneous infusion of atropine abolished the effects of methacholine on the anaphylactic release of TXB2 and cysteinyl-LT. After antigen challenge hearts infused with methacholine, atropine or the combination of both drugs did not exhibit any differences with respect to anaphylactic changes of heart rate or the time course of anaphylactic coronary flow reduction. Thus, in the isolated perfused anaphylactic guinea-pig heart, muscarinic receptor stimulation significantly enhanced the release of the arachidonic acid-derived mediators TXB2 and cysteinyl-LT.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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G Wittmann; P Weinerowski; T Simmet; B A Peskar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Naunyn-Schmiedeberg's archives of pharmacology Volume: 338 ISSN: 0028-1298 ISO Abbreviation: Naunyn Schmiedebergs Arch. Pharmacol. Publication Date: 1988 Nov |
Date Detail:
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Created Date: 1989-06-02 Completed Date: 1989-06-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0326264 Medline TA: Naunyn Schmiedebergs Arch Pharmacol Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 577-81 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, Ruhr-Universität Bochum, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anaphylaxis
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metabolism* Animals Atropine / pharmacology Guinea Pigs Heart / drug effects* Hemodynamics / drug effects Male Methacholine Chloride Methacholine Compounds / pharmacology* Myocardium / metabolism Ovalbumin / pharmacology Receptors, Muscarinic / physiology* SRS-A / analysis, secretion* Thromboxane B2 / analysis, secretion* |
| Chemical | |
Reg. No./Substance:
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0/Methacholine Compounds; 0/Receptors, Muscarinic; 0/SRS-A; 51-55-8/Atropine; 54397-85-2/Thromboxane B2; 62-51-1/Methacholine Chloride; 9006-59-1/Ovalbumin |
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