Document Detail

Effect of multiplicity of infection on Listeria monocytogenes pathogenicity for HeLa and Caco-2 cell lines.
MedLine Citation:
PMID:  8918946     Owner:  NLM     Status:  MEDLINE    
The significance of multiplicity of infection (moi) for invasiveness and intracellular multiplication of Listeria monocytogenes in Caco-2 and HeLa cell monolayers was investigated. A low moi (1:1) resulted in recovery of significantly more L. monocytogenes when these bacteria were used to infect either cell line. At high moi (100:1), the percentage recovery of bacteria was comparatively low, even after extensive invasion and intracellular multiplication. Microscopic analysis of Giemsa- and immunofluorescent-stained infected monolayers revealed extensive cell disruption and exposure of the internalised bacteria to the bactericidal effect of gentamicin. By contrast, a low moi resulted in minimal cytopathic effects and evidence of cell to cell spread by L. monocytogenes was consistently observed in HeLa and J774, but not in Caco-2 cell lines. Nevertheless, the use of HeLa and Caco-2 cell monolayers enabled a clear distinction to be made between invasive (L. monocytogenes) and non-invasive Listeria spp. (L. innocua, L. ivanovii, L. seeligeri, L. grayi, L. welshimeri and L. monocytogenes LLO19). The use of a low moi with HeLa cell monolayers provided a reliable tissue-culture model of infection for L. monocytogenes.
M S Francis; C J Thomas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  45     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-12     Completed Date:  1996-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  323-30     Citation Subset:  IM    
Department of Microbiology and Immunology, University of Adelaide, South Australia.
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MeSH Terms
Caco-2 Cells / microbiology*
Fluorescent Antibody Technique
Hela Cells / microbiology*
Listeria monocytogenes / pathogenicity*

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