| Effect of molecular mass of methoxypoly(ethylene glycol) activated with succinimidyl carbonate on camouflaging pancreatic islets. | |
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MedLine Citation:
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PMID: 20731621 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The surface modification of Langerhans islets by grafting activated poly(ethylene glycol) on to their capsules in order to prevent immune-system stimulation is a novel method in diabetes cell therapy. In the present study, mPEG [methoxypoly(ethylene glycol)] with two molecular masses of 5 and 10 kDa, activated with SC (succinimidyl carbonate), was grafted on to the surface of pancreatic islets at a polymer concentration of 22 mg/ml. It was found that PEGylated islets were viable and active, and no morphological changes on the collagen capsule of islets were observed. The amount of interleukin-2 secretion from lymphocytes co-cultured with islets PEGylated with mPEG-SC of 5 and 10 kDa was 112.12 ± 23.09 pg/ml and 172.75 ± 27.94 pg/ml respectively. Thus mPEG-SC (SC-activated mPEG) with higher molecular mass was more suitable for camouflaging islets from the immune system. |
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Authors:
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Leila Barani; Ebrahim Vasheghani-Farahani; Hamideh Aghajani Lazarjani; Sameereh Hashemi-Najafabadi; Fatemeh Atyabi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and applied biochemistry Volume: 57 ISSN: 1470-8744 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-10-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: England |
Other Details:
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Languages: eng Pagination: 25-30 Citation Subset: IM |
Affiliation:
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Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, 1411713116, Iran. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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