| Effect of mitoxantrone on proliferation dynamics and cell-cycle progression. | |
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MedLine Citation:
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PMID: 19951261 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MTX (mitoxantrone), an anti-tumour antibiotic, is known to cause cell death by intercalating the DNA bases. But how it interferes with the cellular proliferation is not well known. Hence, in the present study, we have tried to evaluate the interaction of this drug using proliferation dynamics to gain a better understanding of MTX's antineoplastic action. Inhibition of proliferation by these drugs was detected by evaluating its effect on cell proliferation and growth curve of the cells. MTX was also found to affect the cell viability and, thereby, cell physiology. Typical apoptotic morphologies such as condensation of nuclei and membrane permeabilization were observed through CLSM (confocal laser scanning microscopy) and fluorescence spectroscopy, which implicates commitment to cell death. Cell-cycle distribution was measured by flow cytometric measurements. The analysis demonstrated significant cell-cycle arrest on MTX treatment. Inhibition of lacZ gene expression was also observed on drug treatment, which implicates its interaction with gene expression. |
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Authors:
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Shahper N Khan; Sunil K Lal; Purnima Kumar; Asad U Khan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioscience reports Volume: 30 ISSN: 1573-4935 ISO Abbreviation: Biosci. Rep. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102797 Medline TA: Biosci Rep Country: United States |
Other Details:
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Languages: eng Pagination: 375-81 Citation Subset: IM |
Affiliation:
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Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Cell Cycle / drug effects* Cell Proliferation / drug effects Colony Count, Microbial Flow Cytometry Gene Expression Regulation, Fungal / drug effects Lac Operon / genetics, physiology Microscopy, Confocal Mitoxantrone / pharmacology* Saccharomyces cerevisiae / cytology, drug effects*, growth & development*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 65271-80-9/Mitoxantrone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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