Document Detail

Effect of minocycline postconditioning and ischemic postconditioning on myocardial ischemia-reperfusion injury in atherosclerosis rabbits.
MedLine Citation:
PMID:  22886964     Owner:  NLM     Status:  In-Data-Review    
This study examined the protective effect of ischemic postconditioning (IPoC) and minocycline postconditioning (MT) on myocardial ischemia-reperfusion (I/R) injury in atherosclerosis (AS) animals and the possible mechanism. Forty male healthy rabbits were injected with bovine serum albumin following feeding on a high fat diet for 6 weeks to establish AS model. AS rabbits were randomly divided into 3 groups: (1) I/R group, the rabbits were subjected to myocardial ischemia for 35 min and then reperfusion for 12 h; (2) IPoC group, the myocardial ischemia lasted for 35 min, and then reperfusion for 20 s and ischemia for 20 s [a total of 3 cycles (R20s/I20s×3)], and then reperfusion was sustained for 12 h; (3) MT group, minocycline was intravenously injected 10 min before reperfusion. The blood lipids, malondialdehyde (MDA), superoxide dismutase (SOD), soluble cell adhesion molecule (sICAM), myeloperoxidase (MPO), and cardiac troponin T (cTnT) were biochemically determined. The myocardial infarction size (IS) and apoptosis index (AI) were measured by pathological examination. The expression of bcl-2 and caspase-3 was detected in the myocardial tissue by using reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the AS models were successfully established. The myocardial IS, the plasma levels of MDA, sICAM, MPO and cTnT, and the enzymatic activity of MPO were significantly decreased, and the plasma SOD activity was significantly increased in IPoC group and MT group as compared with I/R group (P<0.05 for all). The myocardial AI and the caspase-3 mRNA expression were lower and the bcl-2 mRNA expression was higher in IPoC and MT groups than those in I/R group (all P<0.05). It is concluded that the IPoC and MT can effectively reduce the I/R injury in the AS rabbits, and the mechanisms involved anti-oxidation, anti-inflammation, up-regulation of bcl-2 expression and down-regulation of caspase-3 expression. Minocycline can be used as an effective pharmacologic postconditioning drug to protect myocardia from I/R injury.
Conggang Huang; Rui Li; Qiutang Zeng; Yanping Ding; Yongguang Zou; Xiaobo Mao; Wei Hu; Rong Xiong; Ming Li
Related Documents :
22061094 - Esr1 in myocardial infarction.
22427524 - The role of neuregulin/erbb2/erbb4 signaling in the heart with special focus on effects...
3594944 - The possible etiology of the vibratory systolic murmur.
22395114 - Role of metalloproteinases and tissue inhibitors of metalloproteinases during cardiopul...
12878904 - Diagnosing acute myocardial infarction in the setting of left bundle branch block: prev...
19003594 - Lack of consensus in biomarker measurement to diagnose pci-related myocardial infarction.
Publication Detail:
Type:  Journal Article     Date:  2012-08-11
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  32     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  524-9     Citation Subset:  IM    
Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immune safety of a novel oncolytic mutant M1 after administration In Vivo.
Next Document:  Effect of pioglitazone on transdifferentiation of preosteoblasts from rat bone mesenchymal stem cell...