Document Detail

Effect of mild hypothermia on the expression of aquaporin family in cultured rat astrocytes under hypoxic condition.
MedLine Citation:
PMID:  14630348     Owner:  NLM     Status:  MEDLINE    
Aquaporins (AQPs) are a family of water-selective transporting proteins with homology to the major intrinsic protein (MIP) of lens, that increase plasma membrane water permeability in secretory and absorptive cells. In this study, we investigated the effect of mild hypothermia on the expression of AQP4, AQP5 and AQP9 in rat astrocytes cultured under hypoxic conditions. At 37 degrees C, a marked decrease in the expression of AQP4, AQP5 and AQP9 mRNAs was observed. However, at 32 degrees C (mild hypothermia), the expression of AQP5 mRNA was restored to its basal level. Interestingly, under mild hypothermia AQP4 mRNA expression transiently decreased and then increased about two-fold; while AQP9 mRNA expression decreased the same as at 37 degrees C. The changes in the expression of AQP4 and AQP9 proteins were confirmed by Western blot analysis. The restoration of the AQP4 and AQP5 expression at 32 degrees C from the hypoxia-induced decrease at 37 degrees C may play an important role in the reduction of brain edema under hypothermic conditions.
Yoshihito Fujita; Naoki Yamamoto; Kazuya Sobue; Masaaki Inagaki; Hiroaki Ito; Hajime Arima; Tetsuro Morishima; Akinori Takeuchi; Takako Tsuda; Hirotada Katsuya; Kiyofumi Asai
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  47     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-21     Completed Date:  2004-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  437-44     Citation Subset:  IM    
Department of Anesthesiology and Medical Crisis Management, Nagoya City University Graduate School of Medical Sciences, Mizuho-ku, Nagoya 467-8901, Japan.
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MeSH Terms
Anoxia / metabolism*
Aquaporins / biosynthesis*
Astrocytes / physiology*
Blotting, Western
Cells, Cultured
Gene Expression Regulation
Hypothermia, Induced
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Reg. No./Substance:
0/Aquaporins; 0/RNA, Messenger

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