Document Detail

Effect of microtubular or translational inhibitors on general cell protein degradation. Evidence for a dual catabolic pathway.
MedLine Citation:
PMID:  597269     Owner:  NLM     Status:  MEDLINE    
Rat embryo fibroblasts were grown in Eagle's minimal essential medium with 10% serum and cell proteins prelabelled with L-[1-(14)C]leucine, followed by a 24h chase. When transferred to medium deprived of serum these cells showed a 2--3-fold increase in the production of trichloroacetic acid-soluble radioactivity during a 4h observation period. The microtubular poisons vinblastine, vincristine and colchicine partially inhibited this induced proteolysis, but had no effect on the proteolytic rate of cells maintained in medium with 10% serum. A similar discriminating effect on induced proteolysis was observed with cycloheximide, puromycin and insulin. The inhibitory effects of cycloheximide and vinblastine were not additive. These data support the hypothesis that, in addition to the basal turnover of cell proteins, a second mechanism of protein degradation involving cytoplasmic autophagy can be activated by nutritional step-down and is selectively inhibited by agents that interfere with microtubular function and protein synthesis.
J S Amenta; M J Sargus; F M Baccino
Related Documents :
6389849 - Effects of mitochondrial protein synthesis inhibitors on the incorporation of isoleucin...
21184309 - Elisa detection of hazelnut proteins: effect of protein glycation in the presence or ab...
7417519 - Differential effects of proteinase inhibitors and amines on the lysosomal and non-lysos...
15864329 - Phaseococcin, an antifungal protein with antiproliferative and anti-hiv-1 reverse trans...
3734749 - In streptococcus faecium penicillin-binding protein 5 alone is sufficient for growth at...
580529 - Effects of cycloheximide on the ultrastructure of the zona fasciculata of the young rat...
21088489 - Same partners, different dance: involvement of dna replication proteins in centrosome r...
20814869 - Analysis of peptides and proteins in their binding to groel.
9608679 - Lipid-protein complexes as cholesterol pronucleating agents in human bile.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  168     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1977 Nov 
Date Detail:
Created Date:  1978-02-23     Completed Date:  1978-02-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  223-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cells, Cultured
Colchicine / pharmacology
Cycloheximide / pharmacology
Fibroblasts / drug effects,  metabolism,  ultrastructure
Insulin / pharmacology
Protein Biosynthesis
Proteins / metabolism*
Puromycin / pharmacology
Vinca Alkaloids / pharmacology
Reg. No./Substance:
0/Proteins; 0/Vinca Alkaloids; 11061-68-0/Insulin; 53-79-2/Puromycin; 64-86-8/Colchicine; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  S-adenosylmethionine metabolism and its relation to polyamine synthesis in rat liver. Effect of nutr...
Next Document:  Skeletal-muscle sarcolemma from normal and dystrophic mice. Isolation, characterization and lipid co...