Document Detail


Effect of microtubular or translational inhibitors on general cell protein degradation. Evidence for a dual catabolic pathway.
MedLine Citation:
PMID:  597269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat embryo fibroblasts were grown in Eagle's minimal essential medium with 10% serum and cell proteins prelabelled with L-[1-(14)C]leucine, followed by a 24h chase. When transferred to medium deprived of serum these cells showed a 2--3-fold increase in the production of trichloroacetic acid-soluble radioactivity during a 4h observation period. The microtubular poisons vinblastine, vincristine and colchicine partially inhibited this induced proteolysis, but had no effect on the proteolytic rate of cells maintained in medium with 10% serum. A similar discriminating effect on induced proteolysis was observed with cycloheximide, puromycin and insulin. The inhibitory effects of cycloheximide and vinblastine were not additive. These data support the hypothesis that, in addition to the basal turnover of cell proteins, a second mechanism of protein degradation involving cytoplasmic autophagy can be activated by nutritional step-down and is selectively inhibited by agents that interfere with microtubular function and protein synthesis.
Authors:
J S Amenta; M J Sargus; F M Baccino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  168     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1977 Nov 
Date Detail:
Created Date:  1978-02-23     Completed Date:  1978-02-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  223-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Colchicine / pharmacology
Cycloheximide / pharmacology
Fibroblasts / drug effects,  metabolism,  ultrastructure
Insulin / pharmacology
Microtubules
Protein Biosynthesis
Proteins / metabolism*
Puromycin / pharmacology
Rats
Vinca Alkaloids / pharmacology
Chemical
Reg. No./Substance:
0/Proteins; 0/Vinca Alkaloids; 11061-68-0/Insulin; 53-79-2/Puromycin; 64-86-8/Colchicine; 66-81-9/Cycloheximide
Comments/Corrections

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