Document Detail


Effect of methylprednisolone and mechanical loading on canine articular cartilage in explant culture.
MedLine Citation:
PMID:  8731396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to assess the effect of mechanical load on articular cartilage after in vitro corticosteroid exposure. Canine humeral cartilage was equilibrated for 4 days in defined medium with a serum substitute, then exposed to methylprednisolone sodium succinate for 20 h at 0, 0.01 or 1.0 mg/ml. After a drug-free recovery period, the explants were subjected to 0, 1 or 10 mega pascals (MPa) for 1 out every 5 s for 20 min, then incubated with [35S]-sulfate and [3H]-leucine for 4 h to measure proteoglycan and protein synthesis, respectively. When the loading occurred 22 h after drug exposure, proteoglycan synthesis was inhibited and protein synthesis, was unaffected by the drug. Both were stimulated by load, relative to controls. When the loading was delayed until 142 h after drug exposure, there was no biosynthetic response to load whether or not the explant had been exposed to the drug. Proteoglycan and protein synthesis 142 h after 0 or 0.01 mg/ml were unchanged or slightly higher than at 22 h, in explants which did not receive load. In contrast, biosynthesis were strongly inhibited 142 h after 1.0 mg/ml, and there was a 40% loss of proteoglycan content, relative to 22 h controls. If explants receiving 1.0 mg/ml also received heavy (10 MPa) loads 142 h later, there was a 17% reduction in total dry content suggesting severe matrix damage. These in vitro results suggest that articular load can help maintain normal cartilage metabolism after corticosteroid exposure, but also suggest that heavy loading after a sub-clinical dose can cause a marked loss of matrix solids.
Authors:
T Farquhar; R J Todhunter; S L Fubini; N Burton-Wurster; G Lust
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  4     ISSN:  1063-4584     ISO Abbreviation:  Osteoarthr. Cartil.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-09-26     Completed Date:  1996-09-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  55-62     Citation Subset:  IM; S    
Affiliation:
Department of Mechanical Engineering, University of Maryland Baltimore County 21228, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / cytology,  drug effects,  physiology*
Culture Techniques
Dogs
Dose-Response Relationship, Drug
Glucocorticoids / pharmacology*
Humerus / cytology,  drug effects,  physiology*
Methylprednisolone Hemisuccinate / pharmacology*
Proteoglycans / biosynthesis
Weight-Bearing
Grant Support
ID/Acronym/Agency:
AR35664/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/Proteoglycans; 2921-57-5/Methylprednisolone Hemisuccinate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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