Document Detail


Effect of methylglyoxal bis(guanylhydrazone) on polyamine metabolism in normal and regenerating rat liver and rat thymus.
MedLine Citation:
PMID:  4205354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Injections of sublethal doses of methylglyoxal bis(guanylhydrazone), a potent inhibitor of putrescine-activated S-adenosylmethionine decarboxylase in vitro, resulted after a few days in an immense increase in the activity of S-adenosylmethionine decarboxylase in normal and regenerating rat liver and in rat thymus. The increase in the activity of S-adenosylmethionine decarboxylase was at least partly due to a marked lengthening of the half-life of the enzyme. 2. In regenerating liver and thymus there was also a moderate stimulation of the activity of ornithine decarboxylase (EC 4.1.1.17) and a marked accumulation of tissue putrescine. 3. Injection of methylglyoxal bis(guanylhydrazone) into the rat also markedly decreased the activity of diamine oxidase (EC 1.4.3.6) in thymus. 4. In no cases where doses of methylglyoxal bis(guanylhydrazone) close to the LD(50) dose for the rat were used was it possible to lower tissue spermidine content to any significant extent. 5. Methylglyoxal bis(guanylhydrazone) seemed to act as a competitive inhibitor for the substrate S-adenosylmethionine and as an uncompetitive inhibitor for the activator putrescine in the decarboxylation of S-adenosylmethionine in vitro. 6. In the diamine oxidase reaction, with putrescine as the substrate, methylglyoxal bis(guanylhydrazone) was a non-competitive inhibitor for putrescine.
Authors:
E Hölttä; P Hannonen; J Pispa; J Jänne
Related Documents :
3943604 - Degradation of oxalate in rats implanted with immobilized oxalate oxidase.
17393064 - New insights into the structures and functions of human monoamine oxidases a and b.
22961884 - Brunner's gland hyperplasia in the sand rat (psammomys obesus).
25174394 - Sodium ferulate lowers portal pressure in rats with secondary biliary cirrhosis through...
8472784 - Effect of xylose on the synthesis of phosphorylcholine and phosphorylethanolamine in ra...
2135634 - Histological evaluation of biocompatible orthopaedic polymer.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  136     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1974-04-25     Completed Date:  1974-04-25     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  669-76     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aldehydes / pharmacology*
Amidines / pharmacology*
Amine Oxidase (Copper-Containing) / metabolism
Animals
Carbon Radioisotopes
Carboxy-Lyases / antagonists & inhibitors
Female
Glyoxal / pharmacology
Hydrazones / pharmacology
Kinetics
Lethal Dose 50
Liver / enzymology,  metabolism*
Liver Regeneration*
Ornithine
Polyamines / metabolism*
Putrescine
Rats
S-Adenosylmethionine
Spermidine / metabolism
Thymus Gland / enzymology,  metabolism*
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Amidines; 0/Carbon Radioisotopes; 0/Hydrazones; 0/Polyamines; 107-22-2/Glyoxal; 110-60-1/Putrescine; 124-20-9/Spermidine; 29908-03-0/S-Adenosylmethionine; 7006-33-9/Ornithine; EC 1.4.3.6/Amine Oxidase (Copper-Containing); EC 4.1.1.-/Carboxy-Lyases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Biosynthesis of immunoglobulin A (IgA) and immunoglobulin M (IgM). Control of polymerization by J ch...
Next Document:  A serum component related to nonimmunoglobulin amyloid protein AS, a possible precursor of the fibri...