Document Detail


Effect of methotrexate and folinic acid on accumulation of iron in mice.
MedLine Citation:
PMID:  13679009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mouse-model was used to investigate the effect of methotrexate (MTX) and folinic acid on accumulation of iron in young growing mice. Four equal groups of Balb/c young male mice were treated (subcutaneously) with either MTX, or folinic acid, or MTX plus folinic acid, or physiological saline on every second day. After 3 weeks of treatment, liver, spleen, kidney, small intestine, brain, skeletal muscle and heart were removed and analyzed for iron contents using a spectrophotometric method. When the mean values of iron in liver of four groups were compared using one way ANOVA followed by Tukey's HSD test, the group receiving MTX alone was found to have significantly (p=0.004) more accumulation of iron in liver. The group receiving MTX plus folinic acid had iron accumulation in the liver similar to the placebo group. However, the mean values of iron in brain, kidney, small intestine, skeletal muscle, heart and spleen in all the groups, were not found to be statistically different. The data indicate that MTX shifts iron from being utilized in hemoglobin synthesis to liver stores. Folinic acid administration 8 h post-MTX, however, prevents this shift of iron to liver. Decreased levels of iron in plasma in mice treated with MTX alone suggest decreased availability of iron to other tissues for their normal growth and development.
Authors:
M P Iqbal; N Mehboobali; F Sultana; F B Khan; I A Burney; G N Kakepoto
Related Documents :
6303429 - Mössbauer and epr study of the binuclear iron centre in purple acid phosphatase.
2992759 - Electron spin resonance and high pressure liquid chromatographic analysis of melanin in...
21625349 - Caveolae and lipid trafficking in adipocytes.
11457909 - Iron deficiency-associated changes in the composition of the leaf apoplastic fluid from...
2127409 - Release of iron from ferritin by 6-hydroxydopamine under aerobic and anaerobic conditions.
10384799 - Ferrocyanide ingestion may cause false positives in cyanide determination.
17456929 - Use of fumaric acid esters in psoriasis.
57109 - The effect of graded 60 degrees c 1n nitric acid extraction and of deoxyribonuclease di...
8369289 - Structure of novel gangliosides, deaminated neuraminic acid (kdn)-containing glycosphin...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Medical hypotheses     Volume:  61     ISSN:  0306-9877     ISO Abbreviation:  Med. Hypotheses     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-18     Completed Date:  2004-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7505668     Medline TA:  Med Hypotheses     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  444-5     Citation Subset:  IM    
Affiliation:
Department of Biological and Biomedical Sciences, The Aga Khan University, Karachi, Pakistan. perwaiz.iqbal@aku.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Folic Acid Antagonists / pharmacology*
Hemoglobins / metabolism
Iron / metabolism*
Leucovorin / pharmacology*
Male
Methotrexate / pharmacology*
Mice
Mice, Inbred BALB C
Models, Theoretical
Chemical
Reg. No./Substance:
0/Folic Acid Antagonists; 0/Hemoglobins; 58-05-9/Leucovorin; 59-05-2/Methotrexate; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Do cows fed BSE-infected meat and bone meal in the colostrum-producing stage pass on infectious BSE ...
Next Document:  Subjective tinnitus, temporomandibular joint dysfunction, and serotonin modulation of neural plastic...