| Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model. | |
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MedLine Citation:
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PMID: 20576634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Abnormal placentation is a potential mechanism to explain the increased incidence of low birthweight observed after IVF. This study evaluates, in a mouse model, whether the method of conception and embryo transfer affect placentation and fetal development. METHODS: IVF blastocysts (CF1 x B6D2F1/J) were cultured in Whitten's medium (IVF(WM), n = 55) or K modified simplex optimized medium with amino acids (IVF(KAA), n = 56). Embryos were transferred to the uteri of pseudo-pregnant recipients. Two control groups were created: unmanipulated embryos produced by natural mating (in vivo group, n = 64) and embryos produced by natural mating that were flushed from uterus and immediately transferred to pseudo-pregnant recipients (flushed blastocysts, FB group, n = 57). At gestation age 12.5 days, implantation sites were collected and fixed; fetuses and placentas were weighed and their developmental stage (DS) evaluated. Placental areas and vascular volume fractions were calculated; parametric statistics were applied as appropriate. RESULTS: IVF fetuses showed a modest but significant delay in development compared with FB mice (P < 0.05). In addition, IVF conceptuses were consistently smaller than FB (P < 0.05). Importantly, these differences persisted when analyzing fetuses of similar DS. The placenta/fetus ratio was larger in the IVF group (IVF(WM) 0.95; IVF(KAA) = 0.90) than the FB group (0.72) (P < 0.05 for all comparisons). Gross morphology of the placenta and ratio labyrinth/fetal area were equivalent in the IVF and FB groups, as were percentage of fetal blood vessels, maternal blood spaces and trophoblastic components. CONCLUSIONS: In vitro embryo culture affects fetal and placental development; this could explain the lower birthweight in IVF offspring. |
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Authors:
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L Delle Piane; W Lin; X Liu; A Donjacour; P Minasi; A Revelli; E Maltepe; P F Rinaudo |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-06-24 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 25 ISSN: 1460-2350 ISO Abbreviation: Hum. Reprod. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-21 Completed Date: 2010-11-23 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 2039-46 Citation Subset: IM |
Affiliation:
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Obstetric, Gynecology and Reproductive Science Division, University of California San Francisco, San Francisco, CA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Birth Weight Embryo Culture Techniques Embryo Transfer / methods* Female Fertilization in Vitro / adverse effects, methods* Fetal Development* Gestational Age Mice Models, Animal Placenta / growth & development*, pathology Placentation* Pregnancy |
| Grant Support | |
ID/Acronym/Agency:
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1U54HD055764/HD/NICHD NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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