| Effect of metabolism on the anticholinesterase activity of parathion and paraoxon in primary neuron cultures. | |
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MedLine Citation:
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PMID: 20732159 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The anticholinesterase activity of parathion and paraoxon, and the effects of metabolism on this activity, were examined in primary cultures of chick embryo forebrain neurons. Paraoxon was an extremely potent inhibitor of acetylcholinesterase activity, with a median inhibitory concentration (IC(50)) more than 800 times more potent than parathion. Incubation of these two compounds with rat hepatic S-9 fractions, before neuron treatment, dramatically altered their activity, though with opposite effects. Parathion, when preincubated with control S-9 (from saline-treated rats), exhibited increased anticholinesterase activity, whereas paraoxon was much decreased in potency. The relative anticholinesterase activity of these compounds after incubation with the control S-9 fractions was much more predictive of the relative toxicites of these compounds in vivo. Preincubation of these compounds with hepatic S-9 fractions that had been obtained from rats induced with either phenobarbitone or 3-methylcholanthrene, resulted in a decreased activation of parathion and an increased deactivation of paraoxon compared with control S-9. |
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Authors:
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T W Sawyer; M T Weiss; T Dickinson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 6 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 1992 Nov |
Date Detail:
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Created Date: 2010-08-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 569-74 Citation Subset: - |
Affiliation:
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Biomedical Defence Section, Defence Research Establishment Suffield, Box 4000, Medicine Hat, Alberta, Canada T1A 8K6. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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